prehybridization Protocols

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Amino-allyl Labeling (GeneTAC) Protocol - http://www.bcm.edu/mcfweb/?PMID=3551

Category: Microarray Protocols: Amino-allyl Microarray Methods

Protocol for amino-allyl labeling (GeneTAC). Includes: RT Reaction; Nucleotide Mix for one reaction; Coupling; Prehybridization of probe. - [Read Amino-allyl Labeling (GeneTAC) Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for DIG labeling of cDNA probes. Includes: Incorporation DIG during PCR; Estimating the yield of DIG labeled probes; DNA Dot Blotting; Prehybridization and Hybridization. - [Read DIG Labeling of cDNA Probes Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

RNA Whole Mount In Situ Hybridization Protocol - http://genetics.med.harvard.edu/~cepko/protocol/ctlab/ish.ct.htm

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for RNA whole mount in situ hybridization. Includes: Embryo preparation; Prehybridization and Hybridization; Post-hybridization washes, blocking, and antibody incubation; Post-antibody washes; Color development. - [Read RNA Whole Mount In Situ Hybridization Protocol]

Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_208-215.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for whole mount in situ hybridization for the detection of mRNA in Drosophila embryos. Includes: Embryo collection and fixation; Prehybridization treatment; Hybridization; Washing and detection. - [Read Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol]

Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol - http://cobweb.dartmouth.edu/~ambros/worms/14.html

Category: C. elegans Protocols

Protocol for whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. Includes: Collection of Embryos; Permeabilization and fixation of embryos; Prehybridization; Hybridization; Post-hybridization washes; Probe Detection; Double-labeling; Interpretation. - [Read Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol]