preclearing Protocols

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Immunoprecipitation Protocol - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Immunology: Immunoprecipitation Protocols

Protocol for immunoprecipitation. Includes: Cell Lysate Preparation; Cell Lysate Preclearing; Immunoprecipitation; Immunoprecipitation Buffers. - [Read Immunoprecipitation Protocol]

Immunoprecipitation Protocol and Information - http://www.ebioscience.com/ebioscience/appls/IP.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Immunoprecipitation Protocol and Information. IP. Cell Lysate Preparation, preclearing, Immunoprecipitation Buffers. Ebioscience. - [Read Immunoprecipitation Protocol and Information]

Immunoprecipitation: Lysing Bacteria by Sonication Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4532

Category: Bacterial Cell Culture Protocols

In this protocol, bacterial cells are lysed by being subjected to short, intense treatments with ultrasound, which breaks the cell walls and shears the DNA into sizes that will not affect the viscosity of the samples. Note that this method causes some denaturation of the samples. The resulting lysate is ready for preclearing. - [Read Immunoprecipitation: Lysing Bacteria by Sonication Protocol]

Immunoprecipitation: Preclearing the Lysate Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4535

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

To reduce backgrounds and to improve the signal-to-noise ratio, an antibody that does not recognize the antigen being studied can be added to the lysate and processed as for a normal immunoprecipitation. Any nonspecific proteins that might contaminate the final immunoprecipitation step are presumably removed with this irrelevant antibody. - [Read Immunoprecipitation: Preclearing the Lysate Protocol]