This article briefly summarizes the use of this variant of the patch-clamp technique in isolated cardiac myocytes with an emphasis on practical suggestions. - [Read Whole-Cell Voltage Clamp of Isolated Heart Cells]
A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.