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Category: Molecular Biology Protocols: DNA Ligation Protocols
Addition of linkers/adaptors to blunt-end DNA fragments. Ligation of mixture containing DNA and linkers/adaptors. Powell Gannon. Oxford Practical Approach Series. - [Read Addition of linkers/adaptors to blunt-end DNA fragments PDF]
DNA ligation Method, Equipment and reagents, T4 DNA ligase. Gannon and Powell. Oxford Practical Approach Series. - [Read DNA Ligation Protocol PDF]
10 × tailing buffer. Tdt procedure. Terminal transferase and cloning. Powell and Gannon. Oxford Practical Approach Series. - [Read Ligation of DNA fragments by homopolymer tailing]
Category: Real Time PCR
Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves. Dr.Powell. - [Read Real-Time PCR (qPCR) Basics]
Powell and Gannon. Oxford Practical Apporach Series. - [Read Removal of Excess DNA Linkers / Adaptors]
Category: Molecular Biology Protocols: Restriction Enzyme Digestion
Sequential digestion of DNA using two restriction endonucleases. Gannon and Powell. Oxford Practical Approach Series. - [Read Sequential digestion of DNA using two restriction endonucleases]
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