positions Protocols

Search results for: positions

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA. - [Read In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI]

Linkage Analysis Using the NaOH Methylation Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4249

Category: Chromatography Protocols: Gas Chromatography Protocols

Linkage analysis provides information on sugar type, ring size, and substitution positions for each monosaccharide. The method in this protocol, using NaOH as the base, is one of the simpler linkage analysis methods. It requires approximately 1-5 µg of carbohydrate. - [Read Linkage Analysis Using the NaOH Methylation Method Protocol]

Mapping RNA with Nuclease S1 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4053

Category: Nucleic Acid Purification Protocols

Preparations of RNA containing an mRNA of interest are hybridized to a complementary single-stranded DNA probe. At the end of the reaction nuclease S1 is used to degrade unhybridized regions of the probe, and the surviving DNA-RNA hybrids are then separated by gel electrophoresis and visualized by autoradiography or Southern hybridization. Method used to quantitate RNAs, to map the positions of introns, and to identify the locations of 5' and 3' ends of mRNAs on cloned DNA templates. - [Read Mapping RNA with Nuclease S1 Protocol]

Mapping RNA with Ribonuclease and Radiolabeled RNA Probes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4054

Category: Nucleic Acid Modification Protocols: Nucleic Acid Radiolabeling Protocols

Preparations of RNA containing an mRNA of interest are hybridized to a radiolabeled single-stranded RNA probe. The method can be used to quantitate RNAs, to map the positions of introns, and to identify the locations of 5' and 3' ends of mRNAs on cloned DNA templates. - [Read Mapping RNA with Ribonuclease and Radiolabeled RNA Probes Protocol]

Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3946

Category: Bacterial Protocols

Bacterial colonies growing on agar plates are transferred en masse to nitrocellulose filters. The spatial arrangement of colonies on the plates is preserved on the filters. After transfer, the filters are processed for hybridization to an appropriate radiolabeled probe while the original (master) plate is incubated for a few hours to allow the bacterial colonies to regrow in their original positions. - [Read Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol]