polymerization Protocols

Search results for: polymerization

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4115

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

Protocol uses a single thermostable RNA polymerase to perform high-specificity RT-PCR. A high-temperature RT reaction is followed by PCR amplification of the cDNA using a single thermostable poymerase, the GeneAmp AccRT RNA PCR enzyme from Applied Biosystems. The high temperature of the RT reaction enhances the specificity of primer binding and also reduces secondary structure in the template, thereby increasing the efficiency of polymerization. - [Read Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium]

In Vivo Actin Polymerization (cAMP) Protocol - http://www.hopkinsmedicine.org/cellbio/devreotes/invivopoly.htm

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol for the in vivo actin polymerization. - [Read In Vivo Actin Polymerization (cAMP) Protocol]

Recycling Tubulin Protocol - http://mitchison.med.harvard.edu/protocols/recycle.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Recycle tubulin fractions stored at -80¡C after the PC column and store the recycled tubulin in small aliquots for day-to-day use. Generally store recycled tubulin in Injection Buffer (IB) without free GTP. This is done because depolymerization appears to be much better in IB, IB is ideal for microinjections/adding tubulin to extracts, and the absence of free GTP makes polymerization with GMPCPP, a very useful GTP analog that has ~5-10X lower affinity than GTP for tubulin. - [Read Recycling Tubulin Protocol]

Tubulin Polymerization with GTP/GMPCPP/Taxol Protocol - http://mitchison.med.harvard.edu/protocols/poly.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for tubulin polymerization with GTP/GMPCPP/ Taxol. Includes: Solutions & Supplies; Prepolymerization Clarification; GTP Polymerization; Taxol Polymerization; GMPCPP Polymerization; Determining Concentration of GMPCPP/Taxol MTs. - [Read Tubulin Polymerization with GTP/GMPCPP/Taxol Protocol]

Viscosity & Polymeriztion of Microtubules Protocol - http://homepages.gac.edu/~cellab/chpts/chpt9/ex9-5.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for viscosity and polymerization of microtubules. - [Read Viscosity & Polymeriztion of Microtubules Protocol]

Articles (1-1 of 1)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.