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Ancient DNA: Extraction, characterization, molecular cloning, and enzymatic amplification. PDF - http://www.pnas.org/cgi/reprint/86/6/1939

Category: DNA Protocols: DNA Extraction Protocols: Extracting Ancient DNA

The DNA obtained was invariably of a low average molecular size and damaged by oxidative processes, but PCR can be used to amplify and study short mitochondrial DNA sequences thatare of anthropological and evolutionary significance. SVANTE PAABO, PNAS. - [Read Ancient DNA: Extraction, characterization, molecular cloning, and enzymatic amplification. PDF]

Electroporation and RNAi in the Rodent Retina In Vivo and In Vitro Protocol - http://genetics.med.harvard.edu/~cepko/electroporation/PNAS-final.pdf

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol for electroporation and RNAi in the rodent retina in vivo and in vitro. - [Read Electroporation and RNAi in the Rodent Retina In Vivo and In Vitro Protocol]

Electroporation and RNAi in the Rodent Retina In Vivo and In Vitro Protocol - http://genetics.med.harvard.edu/~cepko/electroporation/PNAS-final.pdf

Category: RNAi Technology Protocols

Protocol for electroporation and RNAi in the rodent retina in vivo and in vitro. - [Read Electroporation and RNAi in the Rodent Retina In Vivo and In Vitro Protocol]

Hairpin-bisulfite PCR: Assessing epigenetic - http://staff.washington.edu/miner/hpbspcr/Laird%20et%20al%202004.pdf

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Hairpin-bisulfite PCR will be a powerful tool in studying other processes for which genetic or epigenetic information differs on the two complementary strands of DNA. Laird et al., 2003. PNAS - [Read Hairpin-bisulfite PCR: Assessing epigenetic]

High-resolution SNP mapping by denaturing HPLC - http://www.pnas.org/cgi/content/full/99/16/10575

Category: DNA Protocols: SNP Protocols

High-resolution SNP mapping by denaturing HPLC. A SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. They demonstrate the use of denaturing high-performance liquid chromatography to identify mutations in the candidate genes and to fine-map chromosomal breakpoints. - [Read High-resolution SNP mapping by denaturing HPLC]

Systematic screening of an arrayed cDNA library by PCR PDF - http://www.pnas.org/cgi/reprint/92/6/2209

Category: RNA Protocols: cDNA Libraries Library

Munroe et al PNAS 1995 "..developed a PCR-based method for rapid and effective screening of arrayed cDNA libraries. - [Read Systematic screening of an arrayed cDNA library by PCR PDF]