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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - http://www.mcb.uct.ac.za//corepcr.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - Molecular Biology Techniques Manual - Rybicki - [Read "CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size]

"CORE SAMPLE" PCR - A method to re-PCR unique bands from products of mixed size - http://www.mcb.uct.ac.za//corepcr.htm

Category: PCR Protocols: Standard PCR Protocol

(LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cel - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4107

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

LCM isolates specific cells or tissues from samples mounted on microscope slides. The samples are viewed through a thermoplastic film that is attached to a microcentrifuge tube lid. Localized heat, caused by the application of a laser pulse, fuses the membrane to the cells of interest, which can then be harvested for further analysis. RNA and proteins can be purified from the isolated cells, allowing detailed analysis of gene expression. This protocol is divided into three stages. - [Read (LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cel]

(PI 3-Kinase) Activity Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.assay.a.name.e.Phosphatidyl_Inositol-3_Kinase_Assay_%28PI3%20Kinase%20Assay%29

Category: Signalling Signal Transduction Protocols

Protocol describing (PI 3-Kinase) activity assay. Includes Preparation of Phosphatidylinositol (PI). - [Read (PI 3-Kinase) Activity Assay]

10% Formal Saline Protocol - http://www.nottingham.ac.uk/pathology/protocols/formsal.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 10% formal saine. Includes: 100 litres of 10% Formal Calcium. - [Read 10% Formal Saline Protocol]

12-well Chemotaxis Chamber Protocol - http://www.neuroprobe.com/protocols/AA12.html

Category: Immunology: Chemotaxis

12-well Chemotaxis Chamber Protocol. Preparing the Chamber, Preparing and Adding Responding Cells, Removing, Wiping and Staining the Filter. Neuroprobe - [Read 12-well Chemotaxis Chamber Protocol]

2 Hybrid System TRAFO Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/2HS.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Yeast Two-Hybrid System. TRAFO Solutions. Gietz Lab - [Read 2 Hybrid System TRAFO Protocol]

2-D Polyacrylamide Gel Electrophoresis (Prostate Tissue) - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/2DPage.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

A detailed protocol for 2-D Polyacrylamide Gel Electrophoresis using Prostate Tissue. Molecular Profiling Initiative. - [Read 2-D Polyacrylamide Gel Electrophoresis (Prostate Tissue)]

2-D Polyacrylamide Gel Electrophoresis Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/2DPage.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for 2-D polyacrylamide gel electrophoresis. Includes: 50 ml IEF Lysis Buffer; 10X Electrophoresis Running Buffer (10 L); 30% Acrylamide Stock (1 liter); Separating Acrylamide Gel; 50 ml Equilibration Buffer I; 50 ml Equilibration Buffer II; Transfer Buffer; Sample Preparation; Tissue Processing; Reswelling; Prepare Gradient Acrylamide Gel (9-18%); Transfer and Sequencing of Proteins. - [Read 2-D Polyacrylamide Gel Electrophoresis Protocol]

2D Gel Electrophoresis for Human Plasma Proteome - http://web.archive.org/web/20031115200452/http://iprotocol.mit.edu/protocol/362.htm

Category: Proteomic Protocols: 2-D Gel Electrophoresis

2D Gel Electrophoresis for Human Plasma Proteome. Lee Lab. - [Read 2D Gel Electrophoresis for Human Plasma Proteome]

2x Lysis Buffer Recipe - http://utzlab.stanford.edu/publications/lysisbuffer.doc

Category: Proteomic Protocols: 2-D Gel Electrophoresis

2x Lysis Buffer Recipe. Utz Lab. - [Read 2x Lysis Buffer Recipe]

2X YT Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/2xyt.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for 2X YT Media Recipe (1000 ml). - [Read 2X YT Media (1000 ml) Protocol]

3' Rapid Amplification of cDNA Ends (RACE) Protocol - http://www.nottingham.ac.uk/~mbzspd/methods/3RACE_PCR.html

Category: RNA Protocols: 3' RACE Rapid Amplification of cDNA Ends Protocols

PCR cycle steps, protocol using SuperScript II reverse transcriptase (Life Technologies) and Taq polymerase, Gene specific primer, T17 Adapter primer and an Adapter primer. Dr.Dawson, Nottingham. - [Read 3' Rapid Amplification of cDNA Ends (RACE) Protocol]

3'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4130

Category: PCR Protocols: cDNA Synthesis Protocols

To generate "3'-end" partial cDNA clones, mRNA is reverse-transcribed using a "hybrid" primer (Qtotal, QT) that consists of two mixed bases (GATC/GAC followed by [T]17) and a unique 35-base oligonucleotide sequence (QI-QO). Amplification is then performed using a primer containing part of this sequence (Qouter, Qo) (which now binds to each cDNA at its 3'-end) and a primer derived from the gene of interest, GSP1 (gene-specific primer 1). - [Read 3'-End cDNA Amplification Using Classic RACE Protocol]

3-7 Assay Protocol - http://www.promega.com/tbs/tb323/tb323.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for 3/7 assay. Includes: Detection of Caspase-3 and -7 Activities in Cell-Based Assays; Detection of Caspase-3 or -7 Activity Using Purified Caspases. - [Read 3-7 Assay Protocol]

3-Aminopropyltriethoxysilane Treated Slides Protocol - http://www.nottingham.ac.uk/pathology/protocols/silslid.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for 3-Aminopropyltriethoxysilane treated slides. - [Read 3-Aminopropyltriethoxysilane Treated Slides Protocol]

4% Paraformaldehyde (PFA) Fixative Protocol - http://www.bcm.edu/rosenlab/protocols/pfa.pdf

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 4% paraformaldehyde (PFA) fixative. - [Read 4% Paraformaldehyde (PFA) Fixative Protocol]

4% Paraformaldehyde Protocol - http://www.nottingham.ac.uk/pathology/protocols/paraform.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 4% paraformaldehyde. Includes: 4% PARAFORMALDEHYDE/PBS; 0.4% PARAFORMALDEHYDE/PBS (FOR POST-DIGESTION FIXATION). - [Read 4% Paraformaldehyde Protocol]

5' RACE Protocol For Generation of Seq. Tags - http://baygenomics.ucsf.edu/protocols/comp1/RACE_generation.pdf

Category: RNA Protocols: 5' RACE - Rapid Amplification of cDNA Ends Protocols

Can also use 96-well plates. First Strand cDNA Synthesis, First Size selection, second 2nd strand cDNA synthesis. Bay Area Functional Genomics Consortium, UCSF. - [Read 5' RACE Protocol For Generation of Seq. Tags]

5'-End cDNA Amplification Using Classic RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4131

Category: PCR Protocols: cDNA Synthesis Protocols

To generate "5'-end" partial cDNA clones using classic RACE, the first-strand products are generated by reverse transcription (primer extension) from a known gene-specific primer (GSP-RT). Then, a poly(A) tail is appended using terminal deoxynucleotidyltransferase (Tdt) and dATP. Amplification is carried out using three primers. - [Read 5'-End cDNA Amplification Using Classic RACE Protocol]

5'-End cDNA Amplification Using New RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4132

Category: PCR Protocols: cDNA Synthesis Protocols

New RACE, a variation of RNA ligase-mediated-RACE (RLM-RACE) (Liu and Gorovsky 1993) departs from classic RACE (see 5'-End cDNA Amplification Using Classic RACE) in that an "anchor" primer is attached to the 5'-end of the mRNA before the reverse transcription step; hence the anchor sequence becomes incorporated into the first-strand cDNA if, and only if, the reverse transcription proceeds through the entire length of the mRNA of interest. - [Read 5'-End cDNA Amplification Using New RACE Protocol]

5-Bromo-2-Deoxyuridine (BrdU) and 7-Amino-Actinomycin (7-AAD) Staining for Cell Proliferation Assay - http://www.natureprotocols.com/2007/01/22/5bromo2deoxyuridine_brdu_and_7.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

The incorporation of 5-bromo-2-deoxyuridine (BrdU) in replicating DNA of proliferating cells can be used to measure their proliferation. This protocol allows the identification of cells which have incorporated BrdU by flow cytometry. - [Read 5-Bromo-2-Deoxyuridine (BrdU) and 7-Amino-Actinomycin (7-AAD) Staining for Cell Proliferation Assay]

6-Azauracil Sensitivity Assay for Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4613

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol describes an assay where it requires growing saturated cultures of yeast, counting, and spotting serial dilutions of yeast on both CSM and CSM + 6AU plates. - [Read 6-Azauracil Sensitivity Assay for Yeast Protocol]

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/EthanolFixation.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for 70% ethanol fixation for recovery of DNA, RNA, and protein. - [Read 70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol]

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/EthanolFixation.html

Category: Primary Cell Isolation and Culture Protocols

Protocol for 70% ethanol fixation for recovery of DNA, RNA and protein. - [Read 70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.