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Soybean Cotyledonary-node Agrobacterium-mediated Transformation System - http://www.biotech.unl.edu/transgenic/protocols.html

Category: Plant Biology Protocols

Protocol includes: Seed Sterilization Step; Seed Germination Step; Agrobacterium Inoculum Preparation; Explant Preparation; Co-cultivation Plates; Shoot Initiation Step; Shoot Elongation Step; Rooting Step. - [Read Soybean Cotyledonary-node Agrobacterium-mediated Transformation System]

Top Agar Preparation - http://rothlab.ucdavis.edu/protocols/M11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Prepare Top Agar and pouring plates for top agar. John Roth, U.C Davis. - [Read Top Agar Preparation]

Ultra-Fast Method of DNA Extraction from Neurospora Protocol - http://www.fgsc.net/fgn/camb.html

Category: Fungi Protocols: Neurospora Protocols

Simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation. - [Read Ultra-Fast Method of DNA Extraction from Neurospora Protocol]

Wet-lab protocols - http://eagle.mmid.med.ualberta.ca/private/labnotes/wetlab/index.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

LB plates, LB medium (for 1 liter), Agarose gels. Bart.Hazes Univ Alberta. - [Read Wet-lab protocols]

Yeast Media, Solutions and Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast11.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast Media, Solutions and Stocks Protocols. Includes: Yeast Media; AHC Medium; AHC plates; SD Medium; SD plates; Sorbitol-Agar Medium; Sorbitol-Agar Medium Top Agar; Sporulation Medium Plates; YPD Medium; YPD plates; Synthetic Media Supplements. - [Read Yeast Media, Solutions and Stocks Protocols]

Yeast Plates Protocols - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_plates.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast plates protocols. Includes: Glucose Complete Plates (1 liter); YPD Plates (1 liter); 5-FOA plates (0.5 liter). - [Read Yeast Plates Protocols]

Yeast Two-Hybrid Screen with Library and Bait - http://faculty.virginia.edu/mirmira/resources_files/Protocols/YEAST%20TWO.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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