pipettes Protocols

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DNA Isolation from Onion Cells - http://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.html

Category: DNA Protocols: DNA Extraction Protocols

Allows students to easily isolate chromosomal DNA from onion cells using the same basic tools and methods that scientists use in the lab. A good introduction to using pipettes. Kate Dollard - [Read DNA Isolation from Onion Cells]

Growth of S. cerevisiae and Preparation of DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3298

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes methods for isolation of DNA from a strain of S. cerevisiae carrying a recombinant YAC. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. The method is suitable for preparing DNA that will be used for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that do not require intact high-molecular-weight DNA. - [Read Growth of S. cerevisiae and Preparation of DNA Protocol]

Making Holding Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4394

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes a method for making holding pipettes, to be used for microinjection. - [Read Making Holding Pipettes Protocol]

Making Holding Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4394

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for making holding pipettes, to be used for microinjection. - [Read Making Holding Pipettes Protocol]

Making Injection Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4395

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection. - [Read Making Injection Pipettes Protocol]

Making Injection Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4395

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection. - [Read Making Injection Pipettes Protocol]

Making Pipettes From Hard Glass Capillaries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4354

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for making pipettes from hard glass capillaries. - [Read Making Pipettes From Hard Glass Capillaries Protocol]

Preparation and Loading of Protein Samples for Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4656

Category: Microinjection Protocols: Microinjection of Proteins Protocols

his protocol provides methods for the preparation of protein samples and for loading them into pulled microinjection pipettes. Stock solutions of proteins are thawed, diluted (if desired), centrifuged at high speed to remove aggregates, and kept on ice until loading. Loading into micropipettes can be done using either a "front-loading" or a "backfilling" procedure. - [Read Preparation and Loading of Protein Samples for Microinjection Protocol]

Preparing Siliconized Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4355

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for preparing siliconized pipettes. Such pipettes minimize the loss of embryos or embryonic tissues during transfer. - [Read Preparing Siliconized Pipettes Protocol]