The ABH crosslinker binds to the Fc portion of an antibody molecule, away from the antigen binding site, resulting in a
divalent immunologically active immunoglobulin derivative. Pierce - [Read ABH (p-Azidobenzoyl hydrazide) PDF]
A non-radioactive electrophoretic mobility which works quite well using biotin and streptavidin detection. Non-specific and specific competititor, oligo labeling, Binding Reaction. Pierce - [Read Introduction to the EMSA (Gel Shift) Technique]
PDP-Hydrazide 3-(2-pyridyldithio)propionyl hydrazide is heterobifunctional cleavable crosslinker containing a hydrazide group and pyridyl disulfide residue that react with carbohydrates and sulfhydryls, respectively.
Pierce - [Read PDP-Hydrazide PDF]
Troubleshooting Guide for Chemiluminescent Western Blotting Pierce. High Background, Reverse image on film, Blot glows in the darkroom, Signal duration is less than 8 hours, Weak or no signal, Spots within the protein bands, non-specific bands. - [Read Troubleshooting Guide for Chemiluminescent Western Blotting Pierce]