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Calcium phosphate Tranfection for MDCK cells - http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfection_MDCK.pdf

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Protocol for cell transfection, clone screening and picking cell clones. Bjorkman Group. California I.T - [Read Calcium phosphate Tranfection for MDCK cells]

ES Cell Culture Protocols - ESGRO - http://www.esgro-lif.com/esgro/protocols.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

ES Cell Culture Protocols - EXCELLENT GUIDE. Harvesting and DNA Preparation, Colony Picking, Electroporation, ES Cell Culture using ESGROÂ® and Feeder Cell Layer. ESGRO. - [Read ES Cell Culture Protocols - ESGRO]

Freezing Embryonic Stem (ES) Cells in 96-Well Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4411

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

This protocol describes a method for freezing ES cells in 96-well plates after ES colonies have been isolated by picking. - [Read Freezing Embryonic Stem (ES) Cells in 96-Well Plates Protocol]

Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4415

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

This protocol describes a rapid PCR-based method for identifying targeted ES cell colonies prior to picking. It is based on DNA analysis of a small part of colonies pooled directly from selection plates. Only positive colonies are expanded. - [Read Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking Protocol]

Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4410

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

This protocol describes a method for isolating individual ES cell colonies by picking after they have grown on selective medium. - [Read Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking Protocol]

Picking Bacteriophage lambda Plaques Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3962

Category: Viral Manipulation Protocols: Phage Protocols

General method to pick and store plaques. - [Read Picking Bacteriophage lambda Plaques Protocol]

Picking Single Phage Plaques Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda1.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for the isolation of single bacteriophage lambda plaques to be used for making lysates. - [Read Picking Single Phage Plaques Protocol]

Picking Zygotes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4183

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Zygotes can be identified by their unique morphology. They can be easily separated away from nonmated cells using a micromanipulator. This method provides an alternative to the selection of diploid cells on a medium that prevents the growth of haploid parent cells. - [Read Picking Zygotes Protocol]

Reagents and Guidelines for Long PCR - http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Long PCR Protocols

Long PCR Buffer, Cycle times and temperatures, Picking Primers, Hot Start Polymerases for Long PCR. Harvard-Lipper Center for Computational Genetics. Modified from Cheng et al. - [Read Reagents and Guidelines for Long PCR]

Transposon Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for transposon mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon Mediated Mutagenesis Protocol]

Transposon-Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm

Category: Bacteria Genetics Protocols

Protocol for transposon-mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon-Mediated Mutagenesis Protocol]

Articles (1-2 of 2)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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