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Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3655

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant proteins, constructed in pGEX vectors, are fused to glutathione S-transferase (GST) and can be purified to near homogeneity by affinity chromatography on glutathione-agarose. Bound GST-fusion proteins are readily displaced from the column by elution with buffers containing free glutathione. - [Read Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol]

Purification of GST-fusion proteins E. coli. (pGEX vector) Protocol - http://mullinslab.ucsf.edu/protocols/html/GST_fusion_expression.htm

Category: E Coli Protocols: E coli Protein Protocols

Protocol for the purification of GST-fusion proteins E. coli. (pGEX vector). - [Read Purification of GST-fusion proteins E. coli. (pGEX vector) Protocol]