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C8 Isolation of bovine peripheral blood mononuclear cells by flotation. - http://www.axis-shield.com/densityhome/optiprep/C08.pdf

Category: Cell Biology and Cell Culture

The protocol presented in this Application Sheet uses an alternative strategy to sedimentation on to a density barrier, that is to adjust the density of whole blood to a value just greater than the cells of interest and allow them to float to the surface. - [Read C8 Isolation of bovine peripheral blood mononuclear cells by flotation.]

Culturing Schwann Cells from Adult Peripheral Nerve Protocol - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Rat Neuronal Cell Culture Protocols

Protocol for culturing schwann cells from adult peripheral nerve. - [Read Culturing Schwann Cells from Adult Peripheral Nerve Protocol]

DNA Extraction From Blood Leukocytes - http://geneticslab.topcities.com/DNAextraction.htm

Category: DNA Protocols: DNA Extraction Protocols

DNA extracted from peripheral blood leucocytes using 3mls of whole blood. Modification of a salting out procedure as described by Miller et al., (1988). The DNA Laboratory, Medical School, Malta. - [Read DNA Extraction From Blood Leukocytes]

Human T Cell Activation Protocol - http://www.ebioscience.com/ebioscience/appls/AC145.htm#human

Category: Immunology: T Cell Protocols

Protocol for the Stimulation of human peripheral blood mononuclear cells with anti-human CD3 monoclonal antibody; MTT assay for detection of cellular proliferation. Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. Performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. - [Read Human T Cell Activation Protocol]

Immunofluorescent Staining for Flow Cytometry Protocols - http://www.ebioscience.com/ebioscience/appls/FCS.htm

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocols for immunofluorescent staining for flow cytometry. Includes: Protocol for Staining of Cell Suspensions of Murine Lymphoid Tissue; Protocol for Staining of Human Peripheral Blood; Fluorescent Dyes for Flow Cytometric Analysis - [Read Immunofluorescent Staining for Flow Cytometry Protocols]

Immunofluorescent Staining of Human Peripheral Blood (LWB Procedure) Protocol - http://www.ebioscience.com/ebioscience/appls/FCS.htm#human

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocol for immunofluorescent staining of human peripheral blood (LWB Procedure). - [Read Immunofluorescent Staining of Human Peripheral Blood (LWB Procedure) Protocol]

Isolation of Human NK Subsets Protocol - http://www.natureprotocols.com/2006/08/07/isolation_of_human_nk_subsets.php

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Methodological details about sorting and purification of peripheral blood and decidual human NK subsets. - [Read Isolation of Human NK Subsets Protocol]

MTT Assay for Detection of Cellular Proliferation - http://www.ebioscience.com/ebioscience/appls/AC145.htm#mouse

Category: Immunology: T Cell Protocols

Mouse Protocol: Stimulation of mouse peripheral T cells with plate-bound 145-2C11 monoclonal antibody; MTT assay for detection of cellular proliferation. - [Read MTT Assay for Detection of Cellular Proliferation]

Palmgren's Method for Peripheral Nerve Fibres Protocol - http://www.bristol.ac.uk/vetpath/cpl/palmgren.html

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for Palmgren's method for peripheral nerve fibres. - [Read Palmgren's Method for Peripheral Nerve Fibres Protocol]

Preparation of Cells and Reagents for Flow Cytometry Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C3CC685613447CEC768412FDE0F&objectid=6674EA50F5C3D9A0189B376C7A89BF6B

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A procedure for direct and indirect staining of single-cell suspensions of lymphoid tissue or peripheral blood lymphocytes to detect cell surface membrane antigens is presented. In addition, support protocols present methods for fluorescence labeling of purified antibodies. A protocol for flow cytometric analysis of intracellular antigens in single-cell suspensions is also included. - [Read Preparation of Cells and Reagents for Flow Cytometry Protocols]

Successfully culturing Schwann cells from adult peripheral nerve - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience: Neuron Cell Culture Protocols

Preparation of cultures, Ansselin et al., 1998. Acta Chururgica Austriaca 30. - [Read Successfully culturing Schwann cells from adult peripheral nerve]