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Antigen Design and Sera Purification - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Antisera/Key_Resources/Antigen_Design.html

Category: Immunology: Antigen

Antigen Design and Sera Purification. Custom antisera. Sigma Aldrich. Peptide Selection and Design, Coupling Strategy # Selecting the Protein Carrier, Multiple Antigenic Peptides (MAPs), Choice of Host, Adjuvant, Immunization, & Sera Collection, Antisera Purification, Ammonium Sulfate Precipitation, Protein A/G, Immunoaffinity Purification. - [Read Antigen Design and Sera Purification]

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - http://mitchison.med.harvard.edu/protocols/ab3.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - [Read Coupling Peptides To Resin For Affinity Purification - Claire Walczak.]

Deamidation in Proteins and Peptides - http://www.ionsource.com/Card/Deamidation/mono0001.htm

Category: Mass Spectrometry Protocols

Deamidation in Proteins and Peptides. Informational page by Glen Teshima. RPLC, HIC, Peptide mapping of proteolytic digests (LC-MS), Protein carboxyl methyltransferase (PIMT). - [Read Deamidation in Proteins and Peptides]

Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4549

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for desalting of peptides and protein mixtures by RP-HPLC techniques. The RP-HPLC technique can be used to "desalt" peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. - [Read Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol]

Design, Synthesis, and Characterization of Caged Compounds - http://www.cshprotocols.org/cgi/content/extract/2007/1/pdb.ip25

Category: Microscopy Protocols: Live-Cell Imaging Protocols

When choosing a particular molecule for photoactivation studies, it is necessary to have some structural knowledge of the molecule in order to design an appropriately caged species that will retain its biological inactivity until uncaging is effected. Includes synthesis of caged peptides or proteins. - [Read Design, Synthesis, and Characterization of Caged Compounds]

Epitope Mapping Using Synthetic Biotin-Labeled Peptides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4278

Category: Antibody Protocols: Epitope Mapping Antibody Protocols

A set of overlapping synthetic peptides is synthesized, each corresponding to a small segment of the linear sequence of a protein antigen and arrayed on a solid phase. The panel of solid-phase peptides is then probed with a test antibody, and bound antibody is detected using an enzyme-labeled secondary antibody. This method is very rapid and can be extraordinarily successful. - [Read Epitope Mapping Using Synthetic Biotin-Labeled Peptides Protocol]

In-gel digestion of proteins to be analyzed by mass spectrometry - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/ingeldigest.html

Category: Proteomic Protocols

In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R - [Read In-gel digestion of proteins to be analyzed by mass spectrometry]

Information to Make Antibodies against Peptides - Pierce - http://www.piercenet.com/y/production/producebottom.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Great resource for information. on making antibodies against peptides - Pierce - [Read Information to Make Antibodies against Peptides - Pierce]

Introduction of Caged Peptide/Protein into Cells Using Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4659

Category: Microinjection Protocols: Microinjection of Proteins Protocols

Activation and inactivation of proteins using photoactivation of caged peptides or proteins offer insights into cellular dynamics not achievable using genetic means. The ability to selectively alter the activity of a specific protein at a defined time and location inside a cell allows the correlation of changes in protein activity and cellular behavior. A caged compound, peptide, or protein is prepared by covalently linking it to a photolabile, protecting group. - [Read Introduction of Caged Peptide/Protein into Cells Using Microinjection Protocol]

Magnetic techniques for the isolation and purification of proteins and peptides - http://www.biomagres.com/content/2/1/7

Category: RNA Protocols: RNA-binding Protein Purification

Magnetic techniques for the isolation and purification of proteins and peptides. Safarik et al. 2004 - [Read Magnetic techniques for the isolation and purification of proteins and peptides]

Making antibodies to synthetic peptides - http://medicine.ucsd.edu/hypertension/list_of_protocols/16_antibodies_to_peptides.htm

Category: Peptide Protocols: Antibodies from Peptides Protocols

Protocol for Antibody Creation from a Synthetic Peptide. Antibody from Peptides. Daniel O'Connor. - [Read Making antibodies to synthetic peptides]

Making Antibodies to Synthetic Peptides Protocol - http://medicine.ucsd.edu/hypertension/list_of_protocols/16_antibodies_to_peptides.htm

Category: Antibody Protocols: Antibody Production Protocols

Protocol for making antibodies to synthetic peptides. - [Read Making Antibodies to Synthetic Peptides Protocol]

MALDI Matrices - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/maldimatrices.html

Category: Mass Spectrometry Protocols

MALDI Matrices. Commonly used MALDI matrices for analysis of peptides, proteins, carbohydrates, and nucleic acids using 337 nm or 355 nm UV lasers. All matrices can be used for sample preparation using the Dried Droplet Method whereas only matrices solubl - [Read MALDI Matrices]

Mass spectrometry analysis and identification of proteins - http://www.healthsystem.virginia.edu/internet/biomolec/mass_spectrometry.cfm

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]

PEI Nanoparticles for Targeted Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4451

Category: Gene Delivery Protocols: DNA Nanoparticles Protocols

This protocol describes the preparation of polyethylenimine (PEI)/DNA nanoparticles for targeted gene delivery. This delivery strategy improves the efficiency of gene transfer by enhancing the entry of gene vectors into the desired cells and reducing uptake by nontarget cells. We describe here methods for the conjugation of targeting peptides to PEIs, formation of DNA complexes using the conjugated PEIs or nonconjugated PEIs together with targeting peptides, and cell transfection. - [Read PEI Nanoparticles for Targeted Gene Delivery Protocol]

Peptide Handling Guides - http://www.sigmaaldrich.com/sigma/general%20information/peptide_handling_guide.pdf

Category: Peptide Protocols: Dissolving Handling Peptides

Peptide Handling Guides. Storage Guidelines for Lyophilized Peptides, Strategy for Dissolving Single Peptides, Determining Solubility Characteristics of peptides, Dissolving Approach for Charged Peptides, Dissolving Approach for Hydrophobic/Uncharged Peptides, Guidelines for Dissolving Several Peptides, Peptide Stability and Potential Degradation Pathways, and storage of peptides. Sigma Aldrich. PDF. - [Read Peptide Handling Guides]

Peptide Storage Information - http://www.lerner.ccf.org/services/molecbiotech/peptide.php

Category: Peptide Protocols: Peptide Storage

Great peptide storage information. Lerner. Peptides are provided as lyophilized product that should be stored as dry powder at -20Â°C to -70Â°C in a desiccator, if possible. After lyophilization, peptides retain significant amounts of water. Peptides are slowly degraded particularly the cysteine-containing peptides are oxidized over time at -20Â°C. - [Read Peptide Storage Information]

Ph.D.â„¢-12 Phage Display Peptide Library Kit - http://www.neb.com/nebecomm/ManualFiles/manualE8110.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Ph.D.â„¢-12 Phage Display Peptide Library Kit NEB. Applications for Drug Discovery using Phage Display Libraries of Peptides. - [Read Ph.D.â„¢-12 Phage Display Peptide Library Kit]

Protein Ligand Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4203

Category: Chromatography Protocols: Affinity Chromatography Protocols

Protocol describes the coupling of peptides or proteins to Affi-Gel 10 (Bio-Rad). Affi-Gel 10 is a cross-linked agarose derivatized with N-hydroxysuccinimide (NHS) ester on a 10-atom-long spacer arm. The NHS ester allows spontaneous coupling of proteins via free amino groups. - [Read Protein Ligand Affinity Chromatography Protocol]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4548

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as ß-sheets) that favor supramolecular assembly. - [Read Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol]

Reconstitution of Peptides. - http://www.anaspec.com/html/peptide_tips.html

Category: Peptide Protocols: Dissolving Handling Peptides

Protocol for the reconstitution of Peptides. Anaspec. - [Read Reconstitution of Peptides.]

Redissolve Peptides - http://www.blossombio.com.tw/PDF/FAQ/Peptides%20redissolve.pdf

Category: Peptide Protocols: Dissolving Handling Peptides

Protocol and information on how to redissolve Peptides. - [Read Redissolve Peptides]

The Use of Mass Spectrometry in Proteomics: Database Searching - http://www.cshprotocols.org/cgi/content/extract/2006/27/pdb.ip5

Category: Bioinformatics

Searches are not constrained for only tryptic peptides, and indexed databases (databases only containing tryptic peptides) are not used. In cases where there are very complex mixtures, such as cell lysates, nonspecific cleavages can occur. Therefore, nontryptic peptides would be missed in the database search. - [Read The Use of Mass Spectrometry in Proteomics: Database Searching]

Use of PCR for Quality Control of a Peptide DNA Library Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4137

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Protocol describes the use of PCR to characterize a peptide library encoded in a plasmid vector. In this example, the library was obtained by transforming bacteria with the ligation reaction at the end of Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides. - [Read Use of PCR for Quality Control of a Peptide DNA Library Protocol]