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Peptide Station - http://www.peptidestation.com

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Search Results Protocol Links Sort by: Hits | Alphabetical

Millipore's ZipTip Protocol - http://www.millipore.com/publications.nsf/docs/tn072

Category: Mass Spectrometry Protocols

Millipore's ZipTip Protocol. Intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. Millipore. - [Read Millipore's ZipTip Protocol]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Proteomic protocols and Peptide Extraction - http://www.curie.fr/recherche/themes/equipe-protocoles.cfm/id_equipe/310/lang/_gb.htm

Category: Mass Spectrometry Protocols

Colloidal coomassie blue staining, Destaining, Reduction/Alkylation, Peptide extraction For MALDI-MS analysis and For MALDI-MS analysis protocols and methods. Institut Curie Paris. - [Read Proteomic protocols and Peptide Extraction]

Preparation Of Peptide-KLH Conjugates For Immunization - http://mitchison.med.harvard.edu/protocols/ab1.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Preparation Of Peptide-KLH Conjugates For Immunization. Coupling of Peptide to KLH. And generation of Antibodies using peptide-KLH conjugate - Claire Walczak. - [Read Preparation Of Peptide-KLH Conjugates For Immunization]

Making antibodies to synthetic peptides - http://medicine.ucsd.edu/hypertension/list_of_protocols/16_antibodies_to_peptides.htm

Category: Peptide Protocols: Antibodies from Peptides Protocols

Protocol for Antibody Creation from a Synthetic Peptide. Antibody from Peptides. Daniel O'Connor. - [Read Making antibodies to synthetic peptides]

Tips for Sample Preparation for MALDI mass spectrometry - http://www.cfgbiotech.com/proteomics/sample_prep.htm

Category: Mass Spectrometry Protocols

Tips for Sample Preparation for MALDI mass spectrometry. Sample quantity, Solvent, Protein/Peptide sample handling. U. Albany. CFG - [Read Tips for Sample Preparation for MALDI mass spectrometry]

In-gel digestion of proteins for peptide fingerprint mapping - http://www.bio.vu.nl/vakgroepen/mnb/proteomics/6.in-geldigestion.html

Category: Mass Spectrometry Protocols

Peptide Mapping Analysis Techniques. Dr.Aebersold, Vrije Universiteit Amsterdam - [Read In-gel digestion of proteins for peptide fingerprint mapping]

Deamidation in Proteins and Peptides - http://www.ionsource.com/Card/Deamidation/mono0001.htm

Category: Mass Spectrometry Protocols

Deamidation in Proteins and Peptides. Informational page by Glen Teshima. RPLC, HIC, Peptide mapping of proteolytic digests (LC-MS), Protein carboxyl methyltransferase (PIMT). - [Read Deamidation in Proteins and Peptides]

In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis - http://web.archive.org/web/20010906052645/http://www.pharma.ethz.ch/bmm/protocols/ingeldig.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

In gel trypsin digestion for Mass spectrometry. (According to EMBL Protein and Peptide Group, 1997) - [Read In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis]

Ph.D. Peptide Display Cloning System - NEB - http://www.neb.com/nebecomm/TechBulletinFiles/techbulletinE8101.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Ph.D. Peptide Display Cloning System - NEB. The Ph.D.â„¢ Peptide Display Cloning System facilitates the display of custom peptide libraries on the surface of bacteriophage M13 as coat protein fusions, creating a physical linkage between each displayed pep - [Read Ph.D. Peptide Display Cloning System - NEB]

Purification Of Anti Peptide Antibody - Claire Walczak - http://mitchison.med.harvard.edu/protocols/ab4.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Purification Of Anti Peptide Antibody - Claire Walczak - [Read Purification Of Anti Peptide Antibody - Claire Walczak]

Antigen Design and Sera Purification - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Antisera/Key_Resources/Antigen_Design.html

Category: Immunology: Antigen

Antigen Design and Sera Purification. Custom antisera. Sigma Aldrich. Peptide Selection and Design, Coupling Strategy # Selecting the Protein Carrier, Multiple Antigenic Peptides (MAPs), Choice of Host, Adjuvant, Immunization, & Sera Collection, Antisera Purification, Ammonium Sulfate Precipitation, Protein A/G, Immunoaffinity Purification. - [Read Antigen Design and Sera Purification]

Sequence Specific Retention Calculator - http://hs2.proteome.ca/SSRCalc/SSRCalc.html

Category: HPLC Protocols

Sequence Specific Retention Calculator. A sequence-specific correction factors for prediction of peptide retention in RP-HPLC: application to protein identification by off-line HPLC-MALDI-MS. Oleg Krokhin - [Read Sequence Specific Retention Calculator]

Protein Kinase C Assay - http://cancer.ucsd.edu/newtonlab/Newton%20Web%20PKC%20ASSAY.html

Category: Signalling Signal Transduction Protocols: Protein Kinase Protocols

Protein Kinase C Assay, Newton lab. Using the peptide substrate Ac-FKKSFKL-NH2. Detailed Assay Protocol. - [Read Protein Kinase C Assay]

Peptide Handling Guides - http://www.sigmaaldrich.com/sigma/general%20information/peptide_handling_guide.pdf

Category: Peptide Protocols: Dissolving Handling Peptides

Peptide Handling Guides. Storage Guidelines for Lyophilized Peptides, Strategy for Dissolving Single Peptides, Determining Solubility Characteristics of peptides, Dissolving Approach for Charged Peptides, Dissolving Approach for Hydrophobic/Uncharged Peptides, Guidelines for Dissolving Several Peptides, Peptide Stability and Potential Degradation Pathways, and storage of peptides. Sigma Aldrich. PDF. - [Read Peptide Handling Guides]

Peptide Storage Information - http://www.lerner.ccf.org/services/molecbiotech/peptide.php

Category: Peptide Protocols: Peptide Storage

Great peptide storage information. Lerner. Peptides are provided as lyophilized product that should be stored as dry powder at -20Â°C to -70Â°C in a desiccator, if possible. After lyophilization, peptides retain significant amounts of water. Peptides are slowly degraded particularly the cysteine-containing peptides are oxidized over time at -20Â°C. - [Read Peptide Storage Information]

Peptide Handling and Storage - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Peptides/Key_Resources/Handling___Storage.html

Category: Peptide Protocols: Peptide Storage

In general, peptide solutions are stable for up to a week at 4Â°C. However, if the peptide sequence has inherent instability (Peptide Stability), it might be better to freeze the solution when not in use. Peptide solutions at pH>8 should also be frozen when not in use. Sigma Aldrich. - [Read Peptide Handling and Storage]

Ph.D.â„¢-12 Phage Display Peptide Library Kit - http://www.neb.com/nebecomm/ManualFiles/manualE8110.pdf

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Ph.D.â„¢-12 Phage Display Peptide Library Kit NEB. Applications for Drug Discovery using Phage Display Libraries of Peptides. - [Read Ph.D.â„¢-12 Phage Display Peptide Library Kit]

Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4549

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for desalting of peptides and protein mixtures by RP-HPLC techniques. The RP-HPLC technique can be used to "desalt" peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. - [Read Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol]

Preparation Of Peptide-KLH Conjugates For Immunization - http://mitchison.med.harvard.edu/protocols/ab1.html

Category: Immunology

Checking Peptide Thiol Groups, Coupling of Peptide to KLH. Claire Walczak - [Read Preparation Of Peptide-KLH Conjugates For Immunization]

Peptide Storage Recommendations - http://www.covalab.com/handling/peptide%20handling%20&%20storage.pdf

Category: Peptide Protocols: Peptide Storage

Peptide Storage Recommendations. CovaLab. - [Read Peptide Storage Recommendations]

Testing Rabbit Bleeds By Elisa - http://mitchison.med.harvard.edu/protocols/ab2.html

Category: Immunology

Coupling Peptide to BSA, ELISA with Peptide conjugated to BSA. Claire Walczak - [Read Testing Rabbit Bleeds By Elisa]

Functional Analyses for Site-Specific Phosphorylation of a Target Protein in Cells - http://www.natureprotocols.com/2007/01/10/functional_analyses_for_sitesp.php

Category: Antibody Protocols: Antibody Production Protocols

Novel strategy of immunizing a phosphorylated peptide or a synthetic phosphopeptide, which corresponds to the protein phosphorylated at a targeted residue. Method has been applied to the production of antibodies that can specifically recognize the other types of site-specific protein modification, such as acetylation, methylation, and proteolysis. - [Read Functional Analyses for Site-Specific Phosphorylation of a Target Protein in Cells]

Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4548

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as ß-sheets) that favor supramolecular assembly. - [Read Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol]

CaspaTag Caspase Activity Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/caspatag.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

This assay is based on carboxyfluorescein labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. Includes: Working Dilution of FMK-peptide inhibitors; 1X Working Dilution Wash Buffer; Protocol for Flow Cytometry. - [Read CaspaTag Caspase Activity Protocol]