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How to Make Disposable Spreaders from Long-Nose Pasteur Pipets - http://www.fgsc.net/neurosporaprotocols/How%20to%20make%20disposable%20spreaders%20from%20long-nose%20Pasteur%20pipets%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to make disposable spreaders from long-nose Pasteur pipets. - [Read How to Make Disposable Spreaders from Long-Nose Pasteur Pipets]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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