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Electroporation of ES Cells Protocol - http://www.mshri.on.ca/nagy/Protocols/electrop.htm

Protocol for the electroporation of ES cells. Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for 1-2 electroporations. - [Read Electroporation of ES Cells Protocol]

Freezing and Thawing of Embryonic Stem (ES) Cells Using Cryovials Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4402

Category: Stem Cell Protocols

This protocol describes a method for freezing and thawing ES cells using cryovials. It is important to freeze ES cell stocks as soon as possible to reduce the time that they are in culture. A careful record should be kept of the number of times cells are passaged and the location of the cryovials. - [Read Freezing and Thawing of Embryonic Stem (ES) Cells Using Cryovials Protocol]