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Analysis of Cellular DNA Content by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C8A9C23374B5FAB0610666CCDFB&objectid=6674F58BDEF6DCA5276258FEA4CD534D

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Simple and universally applicable methods for staining fixed cells are presented, as are methods that utilize detergents and proteolytic treatment to permeabilize cells. Additionally, supravital cell staining with Hoechst 33342, which is primarily used for sorting live cells for subsequent culturing based on DNA-content differences, is also described. Also presented are methods for staining of cell nuclei isolated from paraffin-embedded tissues, and deconvolution of DNA-content-frequency... - [Read Analysis of Cellular DNA Content by Flow Cytometry Protocol]

Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol - http://www.animal.ufl.edu/hansen/Protocols/Immunohistochemistry_paraffin.htm

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol used to for immunohistochemistry on paraffin-embedded sections. Based on use of microwave energy to effect antigen retrieval. The immunohistochemistry procedure, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole (AEC) as chromogen. Undoubtedly, other kits or home-made reagents will also work . - [Read Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol]

Chemokine and Interleukin Antibody Protocols - http://www.capralogics.com/Protocols.htm

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Western Blotting chemokine receptors, Immunohistochemistry on Paraffin sections for chemokines. - [Read Chemokine and Interleukin Antibody Protocols]

Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_143-148.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of HPV 11 DNA in paraffin-embedded laryngeal tissue with a DIG-labeled DNA probe. Includes: Probe preparation; Pretreatment of slides; Preparation of tissue sections; Hybridization; Washes; Detection of hybrids. - [Read Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol]

Detection of mRNA on Paraffin Embedded Material of the Central Nervous System with DIG-Labeled RNA - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_164-171.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of mRNA on paraffin embedded material of the central nervous system with DIG-labeled RNA probes. - [Read Detection of mRNA on Paraffin Embedded Material of the Central Nervous System with DIG-Labeled RNA]

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis - http://www.jhc.org/cgi/content/full/46/3/397

Category: Protein Protocols: Protein Extraction From Tissue

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections. Ikedaa et al, 1998 JHC. - [Read Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis]

Fixation. - http://www.dakousa.com/ishbfixation.pdf

Category: Histology Protocols: Histological Fixation

FORMALDEHYDE-BASED FIXATIVES, PARAFFIN-EMBEDDED SECTIONS, BOUINâ€™S SOLUTION, MERCURIC CHLORIDE-BASED FIXATIVES. FARMILO et al. - [Read Fixation.]

HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF - http://www.uchsc.edu/misc/diabetes/derc/protocols/histologyprotocols.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Protocols for cell fixation and tissue fixation. HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block techniques. Embedding Tissue Into Frozen Blocks. Fixing Cells with Fresh Paraformaldehyde. Preparation of Frozen Sections. Immunohistochemistry. Paraffin sections and Frozen sections. DERC Univ. Colorado PDF. - [Read HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF]

Immunofluorescence Staining Protocol - http://www.ihcworld.com/_protocols/general_IHC/immunofl.htm

Category: Immunology: Immunofluorescence Protocols

Protocol for immunofluorescence staining. Includes: Cell Lines; Frozen Sections; Paraffin Sections; Pretreatments of Tissue Sections. - [Read Immunofluorescence Staining Protocol]

Immunohistochemistry Enzyme HRP Staining Protocol - http://www.ihcworld.com/_protocols/general_IHC/immunoenzyme_pod.htm

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry enzyme HRP staining. Includes: Cell Linee; Frozen Sections; Paraffin Sections; Pretreatments of Tissue Sections. - [Read Immunohistochemistry Enzyme HRP Staining Protocol]

Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/immunohistochemistryMWO.html

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry on fixed, paraffin-embedded sections. This method is widely used and applies to the detection of the overwhelming majority of antigens, with few exceptions for which enzymatic retrieval is required. The method uses a strong chelating agent, EDTA. Includes: Double indirect AP; AP Developing solution; Indirect immunohistochemistry with avidin-biotin and HRP; HRP Developing solution. - [Read Immunohistochemistry on Fixed, Paraffin-Embedded Sections Protocol]

Immunohistochemistry Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/Immunohistochemistry.html

Category: Immunohistochemistry Protocols

Protocol describes the application of peroxidase or alkaline phosphatase conjugates in the immunohistochemical labeling of formalin-fixed, paraffin-embedded tissue sections. Includes: Removal of Paraffin and Rehydration; Antigen Retrieval - Unmasking of Antigen; Enzyme retrieval; Microwave retrieval; Inactivation of Endogenous Peroxidase; etc.. - [Read Immunohistochemistry Protocol]

Immunohistochemistry Protocol for γH2AX Detection - http://www.natureprotocols.com/2006/12/06/immunohistochemistry_protocol.php

Category: Immunohistochemistry Protocols

Immunohistochemistry protocol for γH2AX detection (formalin-fixed paraffin-embedded sections). - [Read Immunohistochemistry Protocol for γH2AX Detection]

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti - http://www.jhc.org/cgi/content/full/50/4/449

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Antibodies to Cleaved Caspase 3. Gown et al, 2002 - [Read Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. - http://www.emory.edu/WILCOX/tunel.paraf.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. Wilcox et al. Modified Protocol. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections.]

In Situ Hybridization to Tissue Sections Protocol - http://cmgm.stanford.edu/~vesicle/protocols/in_situ.html

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for in situ hydbridization to tissue sections. Includes: Paraffin Sections; Frozen Sections; Probe preparation; Hybridization and washes; Autoradiography. - [Read In Situ Hybridization to Tissue Sections Protocol]

ISEL in Paraffin-embedded tissues - http://dir.niehs.nih.gov/dirlep/ish/isel1.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: ISEL

Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling). Wijsman et al., 1993. - [Read ISEL in Paraffin-embedded tissues]

Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique - http://www.riedlab.nci.nih.gov/publications/2408.2950_Isolat_Interph_Nuc4Hed204.pdf

Category: Cell Organelle Protocols: Nucleus Protocols

Protocol for the isolation of interphase nuclei from Paraffin Section for FISH using the Hedley Technique. - [Read Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique]

Laser Capture Microdissection Protocols - http://lcm.path.uab.edu/protocols.htm

Category: Histology Protocols: Laser Capture and Microdissection

Preparation of Frozen Tissue for LCM, Preparing Sections from Frozen Tissue for Laser Microdissection, Preparing Sections from Fixed, Paraffin-embedded Tissue for Laser Microdissection Protocols and methods. Laser Microdissection Laboratory. UAB. - [Read Laser Capture Microdissection Protocols]

Paraffin Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/ParaffinEmbedding.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for paraffin embedding. - [Read Paraffin Embedding Protocol]

Paraffin Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/ParaffinEmbedding.html

Category: Primary Cell Isolation and Culture Protocols

Protocol for paraffin embedding. - [Read Paraffin Embedding Protocol]

Paraffin Sectioning for LCM Plant Tissue - http://schnablelab.plantgenomics.iastate.edu/docs/resources/protocols/Paraffin_sectioning_for_LCM.pdf

Category: Histology Protocols: Tissue Sample Sectioning

FIXATION, DEHYDRATION, PARAPLAST INFILTRATION AND SECTIONING PROTOCOL WITH TISSUE COLLECTION PROCEDURE USING THE PALM LASER MICRODISSECTION SYSTEM. Schnable Labs, Plant Genomics. - [Read Paraffin Sectioning for LCM Plant Tissue]

Preparation and LCM of Paraffin Embedded Tissue Sections Protocol - http://dir.nichd.nih.gov/lcm/LCMTAP.htm#II.%20Preparation%20and%20LCM%20of%20Paraffin%20Embedded%20Tissue%20Sections

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for the preparation of LCM of Paraffin embedded tissue sections. Includes: Fixation; Processing, Embedding and Tissue Sectioning; Staining; Laser Transfer; DNA Extraction Protocol of LCM tissue and PCR for DNA analysis; RNA Extraction Protocol of LCM tissue and RT-PCR for mRNA analysis. - [Read Preparation and LCM of Paraffin Embedded Tissue Sections Protocol]

Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2412.2845_Paraff_FrozTiss_Slide_Pretreat.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of paraffin sections and frozen tissue for FISH. - [Read Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol]

Preparation of RNA from Paraffin-Embedded Fixed Tissue Using Trizol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4103

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

The method uses Trizol, a phenol-base reagent produced by Invitrogen. - [Read Preparation of RNA from Paraffin-Embedded Fixed Tissue Using Trizol Protocol]

Articles (1-9 of 9)

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.