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Configuration Column Construction Column Packing for Capillary Liquid Chromatography - http://cshprotocols.cshlp.org/cgi/content/abstract/2007/3/pdb.prot4578

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for configuration, column construction, and column packing for a capillary liquid chromatography system. Protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 µl/min) and gradients required to operate slurry-packed capillary columns. A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to several commercial UV detectors. - [Read Configuration Column Construction Column Packing for Capillary Liquid Chromatography]

Packing an IMAC Column Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4215

Category: Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols

Protocol for packing an IMAC column. Protocol describes a procedure for packing a 30-ml immobilized metal-ion affinity chromatography (IMAC) column with the aid of a pump. - [Read Packing an IMAC Column Protocol]

Packing Capillary Columns for RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4546

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 µl/min) and gradients required to operate slurry-packed capillary columns. A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to a number of commercial UV detectors. - [Read Packing Capillary Columns for RP-HPLC Protocol]

Packing Ceramic Hydroxyapatite Columns for High-Performance Liquid Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/9/pdb.prot4267

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol describes the packing of ceramic hydroxyapatite for HPLC. - [Read Packing Ceramic Hydroxyapatite Columns for High-Performance Liquid Chromatography Protocol]

Preparation of an Ion-Exchange Column Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4195

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for the preparation of ion-exchange chromatography column. Ion-exchange chromatography (IEC) can be used as a crude step in a protein purification scheme, or, with proper preparation, as a high-resolution step. If high resolution is desired, considerable care should be taken during column preparation, choice of IEC media, and column packing. - [Read Preparation of an Ion-Exchange Column Protocol]

Steroid Radioimmunoassay Protocol - http://faculty.washington.edu/jwingfie/protocols.htm

Category: Immunology: Immunoassays

Protocol for steroid radioimmunoassay. Includes: SOLVENT DISTILLATION; PREPARATION OF PLASMA SAMPLES; EXTRACTION OF STEROIDS AND COLUMN PACKING; COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SEPARATION OF BOUND AND FREE COUNTS; DIRECT ASSAYS; SHORT COLUMN CHROMATOGRAPHY. - [Read Steroid Radioimmunoassay Protocol]