oxidized Protocols

Category: Antibody Protocols: Antibody Labeling Protocols

This protocol describes a simple chemical oxidation method for labeling antibodies with iodine. Iodide-125 (supplied as NaI) is oxidized to form iodine-125 (I2), which attacks tyrosyl and histidyl side chains. The iodinated antibodies are easily detected and quantitated using gamma counters or film. They are used primarily in immunoassays, but other techniques can be adapted conveniently to the iodine detection method. - [Read Labeling Antibodies with Iodine Protocol]

Category: Peptide Protocols: Peptide Storage

Great peptide storage information. Lerner. Peptides are provided as lyophilized product that should be stored as dry powder at -20°C to -70°C in a desiccator, if possible. After lyophilization, peptides retain significant amounts of water. Peptides are slowly degraded particularly the cysteine-containing peptides are oxidized over time at -20°C. - [Read Peptide Storage Information]