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Addition of linkers/adaptors to blunt-end DNA fragments PDF - http://www.oup.co.uk/pdf/pas/9v1-7-11.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

Addition of linkers/adaptors to blunt-end DNA fragments. Ligation of mixture containing DNA and linkers/adaptors. Powell Gannon. Oxford Practical Approach Series. - [Read Addition of linkers/adaptors to blunt-end DNA fragments PDF]

Aggregation of ES Cells with Mouse Morula-state Embryos - http://www.oup.co.uk/pdf/pas/13-10-6.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Aggregation of ES Cells with Mouse Morula-state Embryos. Oxford Practical Series Approach. - [Read Aggregation of ES Cells with Mouse Morula-state Embryos]

Combined LacZ staining and in situ hybridization PDF - http://www.oup.co.uk/pdf/pas/13-3-9.pdf

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

Practical Approach Online, Oxford University Press. Combined LacZ staining and in situ hybridization of mouse embryos, beta-galactosidase. - [Read Combined LacZ staining and in situ hybridization PDF]

DNA Ligation Protocol PDF - http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation Method, Equipment and reagents, T4 DNA ligase. Gannon and Powell. Oxford Practical Approach Series. - [Read DNA Ligation Protocol PDF]

Electroporation of Cell Lines With DNA - http://users.path.ox.ac.uk/~ciu/Methods/DNA/electroporation.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

Electroporation of Cell Lines With DNA. Electroporation for the efficient transfection of mammalian cells with DNA. Chu et al. Cellular Immunology Oxford - [Read Electroporation of Cell Lines With DNA]

Embryonic Stem Cell growth media Requirements - Taconic Transgenics - http://www.taconic.com/emerging/ESCells/Growth_Media_solutions.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Reference: Michael P. Matise, Wotjek Auerbach and Alexandra L. Joyner (2000). Gene targeting: a practical approach. Protocol excerpted from Chapter 3, Production of targeted embryonic stem cell clones. Alexandra L. Joyner (ed.), 2nd edition, Oxford Unive - [Read Embryonic Stem Cell growth media Requirements - Taconic Transgenics]

ES Cell Injection Into Mouse Embryos - http://www.oup.co.uk/pdf/pas/13-10-4.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

ES Cell Injection Into Mouse Embryos. Practical Approach Online, Oxford University Press. - [Read ES Cell Injection Into Mouse Embryos]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Injection of ES Cells Into Morulae - http://www.oup.co.uk/pdf/pas/13-10-5.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Injection of ES Cells Into Morulae. Practical Approach Online, Oxford University Press - [Read Injection of ES Cells Into Morulae]

Ligation of DNA fragments by homopolymer tailing - http://www.oup.co.uk/pdf/pas/9v1-7-13.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

10 Ã— tailing buffer. Tdt procedure. Terminal transferase and cloning. Powell and Gannon. Oxford Practical Approach Series. - [Read Ligation of DNA fragments by homopolymer tailing]

Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate - http://www.oup.co.uk/pdf/pas/6-5-3.pdf

Category: DNA Microarray Protocols

Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate. Elder et al., Oxford Practical Approach Series. - [Read Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate]

Removal of Excess DNA Linkers / Adaptors - http://www.oup.co.uk/pdf/pas/9v1-7-12.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

Powell and Gannon. Oxford Practical Apporach Series. - [Read Removal of Excess DNA Linkers / Adaptors]

Sequential digestion of DNA using two restriction endonucleases - http://www.oup.co.uk/pdf/pas/9v1-7-2.pdf

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Sequential digestion of DNA using two restriction endonucleases. Gannon and Powell. Oxford Practical Approach Series. - [Read Sequential digestion of DNA using two restriction endonucleases]

Stripping DNA Microarray array for re-use reusing - http://www.oup.co.uk/pdf/pas/6-5-10.pdf

Category: DNA Microarray Protocols

Reusing DNA Microarrays using Stripping. Elder et al Oxford Practical Series. - [Read Stripping DNA Microarray array for re-use reusing]

Articles (1-2 of 2)

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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