oocyte Protocols

Search results for: oocyte

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Procedures for In Vitro Production of Bovine Embryos - http://www.animal.ufl.edu/hansen/IVF/default.htm

Category: Developmental Biology: Embryology Protocols

Procedures for in vitro production of bovine embryos. Includes: Collection of Ovaries; Oocyte Collection; Preparation of IVF Medium; In Vitro Fertilization; Culture of Embryos. - [Read Procedures for In Vitro Production of Bovine Embryos]

Xenopus Oocyte Transfer Protocol - http://www.xenbase.org/other/static/methods/anti-methods/as-proto.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus oocyte transfer. - [Read Xenopus Oocyte Transfer Protocol]

Articles (1-2 of 2)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

[1-2]