obtaining Protocols

Search results for: obtaining

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

How to Use Asci for Obtaining Double Mutants - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20asci%20for%20obtaining%20double%20mutants%20of%20genes%20that%20show%20epistasis%20of%20are%20phenotypically%20similar.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use asci for obtaining double mutants of genes that show epistasis or are phenotypically similar. - [Read How to Use Asci for Obtaining Double Mutants]

Large-Scale Cell Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662CE6FB2B0DE5496C69D4C4464BA6&objectid=6675A1E8D973D4531201FDB777AF6A84

Category: Cell Culture Protocols

Information on the methods used to cultivate large amounts of cells for the purpose of obtaining an endogenous or recombinant product. - [Read Large-Scale Cell Culture Protocol]

Plant DNA Extraction Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplmy.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibit restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants. The best results are obtained with plants that are two to three weeks post germinated. - [Read Plant DNA Extraction Protocol]

Preparation of Endothelial Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E538DDD4A302A0DF2CDDD9363072C1CA&objectid=6673B460F50B90057960CE47ABB708A4

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large population of highly purified endothelial cells. There are also methods for obtaining endothelial cells from other tissues such as fat, skin, and mucosa. These methods require special care and generate smaller populations of cells. - [Read Preparation of Endothelial Cells Protocol]