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ELISA (Enzyme-Linked ImmunoadSorbent Assay) - http://www.nottingham.ac.uk/~mbzspd/methods/ELISA.html

ELISA (Enzyme-Linked ImmunoadSorbent Assay) Method and technique. Jill Fergusson, Department of Biochemistry, University of Nottingham, - [Read ELISA (Enzyme-Linked ImmunoadSorbent Assay)]

Enzyme Histochemical Stains Protocol - http://www.nottingham.ac.uk/pathology/protocols/nse.html

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for enzyme histochemical stain. - [Read Enzyme Histochemical Stains Protocol]

Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

General Stain for Toludine Blue Protocol - http://www.nottingham.ac.uk/pathology/protocols/tolblue.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol for toludine blue general stain. - [Read General Stain for Toludine Blue Protocol]

GIEMSA for Helicobacter pylori Protocol - http://www.nottingham.ac.uk/pathology/protocols/giemshel.html

Category: Neuroscience Protocols: Histology Stains Protocols: Giemsa Stains Protocols

Protocol for GIEMSA for Helicobacter pylori. - [Read GIEMSA for Helicobacter pylori Protocol]

Gills Haematoxylin Stain Solution Protocol - http://www.nottingham.ac.uk/pathology/protocols/gills.html

Category: Histology Stains Protocols

Protocol for Gills Haematoxylin stain solution. - [Read Gills Haematoxylin Stain Solution Protocol]

Gram Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/gram.html

Category: Staining Protocols: Gram Staining Protocols

Protocol for gram stain. Includes: Hucker-conn ammonium oxalate - Crystal violet; Weigerts Iodine; 0.1% Nuclear fast red. - [Read Gram Stain Protocol]

Haematoxylin and Eosin Technique Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/hande.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin and eosin technique stain. - [Read Haematoxylin and Eosin Technique Stain Protocol]

Haematoxylin Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/harris.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin stain. - [Read Haematoxylin Stain Protocol]

Haematoxylin Stain Solution Protocol - http://www.nottingham.ac.uk/pathology/protocols/carazz.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin stain solution. - [Read Haematoxylin Stain Solution Protocol]

Haematoxylin Van Gieson Tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/hvg.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin van gieson tissue stain. - [Read Haematoxylin Van Gieson Tissue Stain Protocol]

High Iron Diamine Stain for Sulphomucins Protocol - http://www.nottingham.ac.uk/pathology/protocols/hidiam.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol stain for sulphomucins- high iron diamine stain. - [Read High Iron Diamine Stain for Sulphomucins Protocol]

Home-made Taq Polymerase Purification - http://www.nottingham.ac.uk/~mbzspd/methods/Home_made_Taq_polyme.html

Category: PCR Protocols

PCR polymerase costs can be high. If you are willing to work, you can produce bacteria containing the clone. It appears to produce lots of Taq and is quite stable. The proceedure takes 4 days start to (15 000 units of Taq) finish. The Taq also appears ver - [Read Home-made Taq Polymerase Purification]

In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters - http://www.nottingham.ac.uk/~mbzspd/methods/In_vitro_RNA_synthes.html

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

RNA, in vitro synthesis using T3 T7 promoters. Dr. Dawson Univ. Nottingham. - [Read In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters]

Indirect Peroxidase Technique Fixed tissue Protocol - http://www.nottingham.ac.uk/pathology/protocols/indfixed.html

Category: Immunohistochemistry Protocols

Protocol for indirect peroxidase technique fixed tissue. - [Read Indirect Peroxidase Technique Fixed tissue Protocol]

Indirect Peroxidase Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/indfroz.html

Category: Immunohistochemistry Protocols

Protocol for indirect peroxidase technique. Includes: Sections: Thickness-Between 5 and 10 microns. Pick up on chromic acid etched, poly-L-lysine or chrome alum/gelatine coated slides. Dry at room temperature overnight (not longer than 72 hours). - [Read Indirect Peroxidase Technique Protocol]

Lactase Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/lactase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for lactase histochemical stain. - [Read Lactase Histochemical Stain Protocol]

Leucine Amino Peptidase Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/lapep.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for leucine amino peptidase histochemical stain. - [Read Leucine Amino Peptidase Histochemical Stain Protocol]

Library cDNA Synthesis - http://www.nottingham.ac.uk/~mbzspd/methods/Library_cDNA_Synthes.html

Category: RNA Protocols: cDNA Libraries Library

Library cDNA Synthesis. 1rst and 2nd strand cDNA synthesis. Dr. Simon Dawson - [Read Library cDNA Synthesis]

Masson Fontana Protocol - http://www.nottingham.ac.uk/pathology/protocols/mfontana.html

Category: Neuroscience Protocols: Histology Stains Protocols: Amine Stains Protocols

Protocol for masson fontana. - [Read Masson Fontana Protocol]