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Competitive ELISA Procedure - http://www.nottingham.ac.uk/~mbzspd/methods/Competitive_ELISA.html

Category: ELISA Protocols

Competitive ELISA Protocol. Jill Fergusson, University of Nottingham. - [Read Competitive ELISA Procedure]

ELISA (Enzyme-Linked ImmunoadSorbent Assay) - http://www.nottingham.ac.uk/~mbzspd/methods/ELISA.html

Category: ELISA Protocols

ELISA (Enzyme-Linked ImmunoadSorbent Assay) Method and technique. Jill Fergusson, Department of Biochemistry, University of Nottingham, - [Read ELISA (Enzyme-Linked ImmunoadSorbent Assay)]

3' Rapid Amplification of cDNA Ends (RACE) Protocol - http://www.nottingham.ac.uk/~mbzspd/methods/3RACE_PCR.html

Category: RNA Protocols: 3' RACE Rapid Amplification of cDNA Ends Protocols

PCR cycle steps, protocol using SuperScript II reverse transcriptase (Life Technologies) and Taq polymerase, Gene specific primer, T17 Adapter primer and an Adapter primer. Dr.Dawson, Nottingham. - [Read 3' Rapid Amplification of cDNA Ends (RACE) Protocol]

4% Paraformaldehyde Protocol - http://www.nottingham.ac.uk/pathology/protocols/paraform.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 4% paraformaldehyde. Includes: 4% PARAFORMALDEHYDE/PBS; 0.4% PARAFORMALDEHYDE/PBS (FOR POST-DIGESTION FIXATION). - [Read 4% Paraformaldehyde Protocol]

Home-made Taq Polymerase Purification - http://www.nottingham.ac.uk/~mbzspd/methods/Home_made_Taq_polyme.html

Category: PCR Protocols

PCR polymerase costs can be high. If you are willing to work, you can produce bacteria containing the clone. It appears to produce lots of Taq and is quite stable. The proceedure takes 4 days start to (15 000 units of Taq) finish. The Taq also appears ver - [Read Home-made Taq Polymerase Purification]

Poly-L-Lysine Coated Slides Protocol - http://www.nottingham.ac.uk/pathology/protocols/pll.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for poly-L-lysine coated slides. Includes: SKI STAINING MACHINE; SHANDON ELLIOT CAROUSEL. - [Read Poly-L-Lysine Coated Slides Protocol]

Alcian Blue Staining Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/alcblue.html

Category: Neuroscience Protocols: Histology Stains Protocols: Mucin Stains Protocols

Protocol for alcian blue staining technique. - [Read Alcian Blue Staining Technique Protocol]

In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters - http://www.nottingham.ac.uk/~mbzspd/methods/In_vitro_RNA_synthes.html

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

RNA, in vitro synthesis using T3 T7 promoters. Dr. Dawson Univ. Nottingham. - [Read In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters]

Alkaline Phosphatase (APAAP) Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/apap.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for alkaline phosphatase (APAAP) technique. Includes: Fixation; Technique; Substrate Solution. - [Read Alkaline Phosphatase (APAAP) Technique Protocol]

Library cDNA Synthesis - http://www.nottingham.ac.uk/~mbzspd/methods/Library_cDNA_Synthes.html

Category: RNA Protocols: cDNA Libraries Library

Library cDNA Synthesis. 1rst and 2nd strand cDNA synthesis. Dr. Simon Dawson - [Read Library cDNA Synthesis]

Haematoxylin and Eosin Technique Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/hande.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin and eosin technique stain. - [Read Haematoxylin and Eosin Technique Stain Protocol]

SECTIONING RESIN EMBEDDED TISSUE - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Histology Protocols: Tissue Sample Sectioning

SECTIONING RESIN EMBEDDED TISSUE. MAKING LATTA-HARTMANN KNIVES and Sectioning Protocols for Histology. University of Nottingham Medical School Division of Histopathology. James Lowe. - [Read SECTIONING RESIN EMBEDDED TISSUE]

10% Formal Saline Protocol - http://www.nottingham.ac.uk/pathology/protocols/formsal.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 10% formal saine. Includes: 100 litres of 10% Formal Calcium. - [Read 10% Formal Saline Protocol]

Elastic Picro Sirius Red Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/epsr.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for elastic picro sirius red connective tissue stain. - [Read Elastic Picro Sirius Red Stain Protocol]

Cresyl Fast Violet Protocol - http://www.nottingham.ac.uk/pathology/protocols/cfvmast.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for cresyl fast violet. - [Read Cresyl Fast Violet Protocol]

Gram Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/gram.html

Category: Staining Protocols: Gram Staining Protocols

Protocol for gram stain. Includes: Hucker-conn ammonium oxalate - Crystal violet; Weigerts Iodine; 0.1% Nuclear fast red. - [Read Gram Stain Protocol]

Anti Peroxidase Technique - http://www.nottingham.ac.uk/pathology/protocols/papihc.html

Category: Immunohistochemistry Protocols

Anti peroxidase technique. Includes: DAB Solution. - [Read Anti Peroxidase Technique]

Alkaline Phosphatase (APAAP) Technique - http://www.nottingham.ac.uk/pathology/protocols/apap.html

Category: Signalling Signal Transduction Protocols

Technique for Alkaline Phosphatase (APAAP). Techniques includes information on: Cytological Preparations, Fixation, Technique, Results, Substrate Solution and 0.005M Tris/HC1 Saline Buffer pH 7.6. - [Read Alkaline Phosphatase (APAAP) Technique]

3-Aminopropyltriethoxysilane Treated Slides Protocol - http://www.nottingham.ac.uk/pathology/protocols/silslid.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for 3-Aminopropyltriethoxysilane treated slides. - [Read 3-Aminopropyltriethoxysilane Treated Slides Protocol]

Stain for Fibrin M.S.B Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/msb.html

Category: Histology Stains Protocols

Protocol stain for fibrin: M.S.B technique. - [Read Stain for Fibrin M.S.B Technique Protocol]

Decalcification Protocol - http://www.nottingham.ac.uk/pathology/protocols/decal.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for decalcification. - [Read Decalcification Protocol]

Haematoxylin Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/harris.html

Category: Neuroscience Protocols: Histology Stains Protocols: Hematoxylin Eosin Staining Protocols

Protocol for haematoxylin stain. - [Read Haematoxylin Stain Protocol]

Adenosine Triphosphatase ATPASE Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/atpase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for adenosine triphosphatase ATPASE histochemical stain. - [Read Adenosine Triphosphatase ATPASE Histochemical Stain Protocol]

Cytochrome Oxidase Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/cox.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for cytochrome oxidase technique, enzyme histochemical stains. - [Read Cytochrome Oxidase Technique Protocol]

Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

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