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Big Dye Protocols and Notes - Cosmid, BAC, BAC, Fosmid Templates - http://www.genome.ou.edu/big_dyes_cos_bac_pac_fos.html

Category: DNA Protocols: DNA Sequencing Protocols: Big Dye Protocols

Big Dye protocols and notes for: cosmid, BAC, BAC, Fosmid Templates. - [Read Big Dye Protocols and Notes - Cosmid, BAC, BAC, Fosmid Templates]

Cohesive End Ligation - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/ligation.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation protocol with excellent notes and tips. Chen Lab, Univ. College London. - [Read Cohesive End Ligation]

Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates - http://www.genome.ou.edu/big_dyes_cos_bac_pac_fos.html

Category: BACs Bacterial Artificial Chromosome Protocols

Sequencing conditions tested for the ABI Big-Dye terminators (PE-ABI #4303150 for the 1000 reaction kit - Description: TF,KIT BTD RR-1000) with various templates. Important to quantitate all templates by agarose gel electrophoresis vs size and concentration standards and do a few tests with different template concentrations to determine the optimal conditions for your reactions. Several conditions are given. - [Read Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates]

ES Cell Culture Presentation: Notes from the Monash IRD Lab. PPT - http://www.jax.org/courses/archives/2003/stem03_pera.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

ES Cell Culture Presentation: Notes from the Monash IRD Lab. PPT. Powerpoint slides. Martin Pera.Monash University. - [Read ES Cell Culture Presentation: Notes from the Monash IRD Lab. PPT]

General Confocal Protocols - http://www.rci.rutgers.edu/~rsharma1/protocols.html

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

General Confocal Protocols. Confocal Startup Protocol, Confocal Clean-Up Protocol, Confocal Printing Protocol.CONVENTIONAL MICROSCOPY NOTES. Piscataway, New Jersey. Rutgers University - [Read General Confocal Protocols]

In Vitro Transcription Protocol - http://www.cvmbs.colostate.edu/mip/wiluszlab/protocols/invitrotrans.htm

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription. Includes information on: Hot transcription reactions, notes and troubleshooting. - [Read In Vitro Transcription Protocol]

Lowry â€“ Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/loury.html

Category: Protein Protocols: Lowry Protein Assay

Lowry â€“ Protein Determination. Quantitation of total protein using the lowry protein assay. Dr. Mario Lebendiker. The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. Method materials and notes for the Lowry assay. - [Read Lowry â€“ Protein Determination]

Yeast Chromatin Immunoprecipitation (ChIP) - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/hahnlab_ChIP_method.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. - [Read Yeast Chromatin Immunoprecipitation (ChIP)]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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