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Detection of si/miRNA in C. elegans by Northern Blot Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4320

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol uses northern blot technology to detect short interfering RNAs (siRNAs) or micro-RNAs (miRNAs) in RNA preparations from Caenorhabditis elegans. - [Read Detection of si/miRNA in C. elegans by Northern Blot Protocol]

Detection of siRNA in C. elegans Using Rnase Protection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4319

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol uses Rnase protection to detect short interfering RNAs (siRNAs) in RNA preparations from Caenorhabditis elegans. SiRNAs can also be detected by northern blot. However, the Rnase protection assay seems to be more sensitive. - [Read Detection of siRNA in C. elegans Using Rnase Protection Protocol]

Northern Blot Analysis of mRNA From Mammalian Polyribosomes Protocol - http://www.natureprotocols.com/2006/09/15/northern_blot_analysis_of_mrna.php

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

The protocol consists of a method for the generation of cytoplasmic extracts from mammalian cells (in this case, 293T cells) without the disruption of polyribosomes, the separation of ribosomal components and polyribosomes by sucrose gradient centrifugation, the isolation of mRNA from these fractions, and detection of mRNA by Northern blot analysis. - [Read Northern Blot Analysis of mRNA From Mammalian Polyribosomes Protocol]

Northern blot Protocol for the Detection of RNA in Neurospora - http://www.fgsc.net/neurosporaprotocols/Northern%20edited%20version.pdf

Category: Fungi Protocols: Neurospora Protocols

Northern blot protocol for the detection of RNA in Neurospora. Includes: Extract RNA from tissue powder; Electropheresis and transfer RNA; Preparing the membrane for probing and preparation of a riboprob.; Hybridization. - [Read Northern blot Protocol for the Detection of RNA in Neurospora]

Northern Hybridization Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3723

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for northern hybridization. Protocol describes how to carry out northern hybridization at high stringency in phosphate-SDS-buffers. Although a wide variety of formats are available, hybridization is usually performed in heat-sealable bags, roller bottles, or plastic boxes, as described here. - [Read Northern Hybridization Protocol]

RNA (Northern) Blot Analysis Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/RNAhybs.pdf

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for RNA (northern) blot analysis. - [Read RNA (Northern) Blot Analysis Protocol]

Separation of RNA According to Size: Electrophoresis of Glyoxylated RNA through Agarose Gels - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4057

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Separation of RNAs according to size is the first stage in northern blotting and hybridization. The method described in this protocol uses glyoxal to denature the RNA, ethidium bromide to stain it, and agarose gel electrophoresis to separate the resulting glyoxal-RNA-ethidium adducts. - [Read Separation of RNA According to Size: Electrophoresis of Glyoxylated RNA through Agarose Gels]

Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4050

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Separation of RNAs according to size is the first stage in northern blotting and hybridization. The method described in this protocol uses formaldehyde to denature the RNA, ethidium bromide to stain it, and electrophoresis through agarose gels containing 2.2 M formamide to separate the resulting formaldehyde-RNA-ethidium adducts. - [Read Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde]