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Annexin V Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/annexin.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Different cell types vary in their phosphatidylserine (PS) content, along with the amount of PS exposure on the cell surface after cell death. This protocol is a guideline for getting started, however it may be necessary to adjust the concentration of the Annexin V-FITC. - [Read Annexin V Protocol for Flow Cytometry]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for calcium flux analysis. Includes: Reagent Preparation; Sample Preparation; Cell Surface Staining; Instrument Set up; Optimizing the Instrument Settings; Recording Data to Apply Compensation; Recording Experimental Data. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

Calcium Flux Analysis Protocol for Flow Cytometry - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/calcium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for calcium flux analysis for flow cytometry. - [Read Calcium Flux Analysis Protocol for Flow Cytometry]

CaspaTag Caspase Activity Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/caspatag.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

This assay is based on carboxyfluorescein labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. Includes: Working Dilution of FMK-peptide inhibitors; 1X Working Dilution Wash Buffer; Protocol for Flow Cytometry. - [Read CaspaTag Caspase Activity Protocol]

DNA Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/dna.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for DNA analysis by flow cytometry. - [Read DNA Analysis by Flow Cytometry Protocol]

FASC Analysis of Cell Cycle using BrdU and PI Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/fasc.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocols for FASC analysis of cell cycle using BrdU and PI. Includes: Labeling of Cells with BrdU; Collecting the Cells; Processing the Cells. - [Read FASC Analysis of Cell Cycle using BrdU and PI Protocol]

Immunofluorescent Staining of Rat Thymocytes Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/immunofluorescent.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for the immunofluorescent staining of rat thymocytes. - [Read Immunofluorescent Staining of Rat Thymocytes Protocol]

ISEL in Paraffin-embedded tissues - http://dir.niehs.nih.gov/dirlep/ish/isel1.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: ISEL

Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling). Wijsman et al., 1993. - [Read ISEL in Paraffin-embedded tissues]

Membrane Potential Analysis by Flow Cytometry Protoocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/membrane.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for membrane potential analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Membrane Potential Analysis by Flow Cytometry Protoocol]

PARP PE Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/parp.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for PARP PE. - [Read PARP PE Protocol]

Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transferase - http://dir.niehs.nih.gov/dirlep/ish/tunel1.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: ISEL

Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transferase (TdT). Gavrieli et al., 1993. NIEHS. - [Read Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transferase]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for sodium and potassium analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Sodium and Potassium Analysis by Flow Cytometry Protocol]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols