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A Safe Method of Extracting DNA from Coccidioides immitis Protocol - http://www.fgsc.net/fgn42/burt.html

Category: Fungi Protocols: Fungi Genetics Protocols

Protocol describes a safe and convenient method of extracting DNA from Coccidioides immitis fungi in which the culture is killed by steaming, allowing removal from the containment facilities, as soon as possible. The method was first developed with the non-pathogen Neurospora crassa, has worked well for both C. immitis and H. capsulatum, and should be useful for extracting DNA from any pathogenic fungus. - [Read A Safe Method of Extracting DNA from Coccidioides immitis Protocol]

Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae - http://www.fgsc.net/neurosporaprotocols/Cryofixation_NC.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for cryofixation and freeze substitution for ultrastructural studies of Neurospora crassa hyphae. - [Read Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae]

Generation of Transformable Spheroplasts Protocol - http://www.fgsc.net/fgn39/royer.html

Category: Fungi Protocols: Neurospora Protocols

Protocol for generation of transformable spheroplasts from mycelia, macroconidia, microconidia and germinating ascospores of Neurospora crassa. - [Read Generation of Transformable Spheroplasts Protocol]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

How and Where to Obtain Information on Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20and%20where%20to%20obtain%20information.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how and where to obtain information on Neurospora. - [Read How and Where to Obtain Information on Neurospora]

How to Assay Light Responses in Neurospora - http://www.fgsc.net/neurosporaprotocols/lightresponses.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to assay light responses in Neurospora. - [Read How to Assay Light Responses in Neurospora]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Certify that Neurospora is Nonhazardous - http://www.fgsc.net/neurosporaprotocols/How%20to%20certify%20that%20Neurospora%20final%20.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to certify that Neurospora is nonhazardous. - [Read How to Certify that Neurospora is Nonhazardous]

How to Create Gene Knockouts in Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20to%20create%20gene%20knockouts%20in%20Neurospora.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to create gene knockouts in Neurospora. - [Read How to Create Gene Knockouts in Neurospora]

How to Demonstrate Barrage in Neurospora - http://www.fgsc.net/neurosporaprotocols/How%20to%20demonstrate%20barrage%20with%20figure.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to demonstrate barrage in Neurospora. - [Read How to Demonstrate Barrage in Neurospora]

How to Isolate Nondenatured Protein Extracts from Neurospora for Western Analysis - http://www.fgsc.net/neurosporaprotocols/How%20to%20isolate%20nondenatured%20protein%20extracts%20from%20Neurospora%20for%20western%20analysis.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to isolate nondenatured protein extracts from Neurospora for western analysis. - [Read How to Isolate Nondenatured Protein Extracts from Neurospora for Western Analysis]

How to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium Protocol - http://www.fgsc.net/neurosporaprotocols/How%20to%20Isolate%20Tightly-Coupled%20Mitochondria.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol on how to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium. A Fast and Reproducible Procedure - [Read How to Isolate Tightly-Coupled Mitochondria from Neurospora crassa mycelium Protocol]

How to Make Heterokaryons in Neurospora crassa - http://www.fgsc.net/neurosporaprotocols/How%20to%20make%20heterokaryons%20in%20Neurospora%20crassa.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to make heterokaryons in Neurospora crassa. - [Read How to Make Heterokaryons in Neurospora crassa]

How to Procure Neurospora Strains for Research or Teaching - http://www.fgsc.net/neurosporaprotocols/How%20to%20procure%20Neurospora%20strains%20for%20research%20and%20teaching.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to procure Neurospora strains for research or teaching. - [Read How to Procure Neurospora Strains for Research or Teaching]

How to Test Heat, Osmotic, and Oxidative Tolerance of Neurospora Conidia and Young Mycelia - http://www.fgsc.net/neurosporaprotocols/How%20to%20test%20thermal%20osmotic%20and%20oxidative%20stress%20tolerance.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to test heat, osmotic, and oxidative tolerance of Neurospora conidia and young mycelia. - [Read How to Test Heat, Osmotic, and Oxidative Tolerance of Neurospora Conidia and Young Mycelia]

How to Transform Neurospora crassa by Electroporation - http://www.fgsc.net/neurosporaprotocols/How%20to%20transform%20Nc%20by%20electroporation.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to transform Neurospora crassa by electroporation. - [Read How to Transform Neurospora crassa by Electroporation]

In Vitro System to Analyze Light Signal Transduction in Neurospora crassa - http://www.fgsc.net/neurosporaprotocols/How%20to%20analyze%20light%20signal%20transduction%20in%20vitro.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for an in vitro system to analyze light signal transduction in Neurospora crassa - [Read In Vitro System to Analyze Light Signal Transduction in Neurospora crassa]

Lipofectin Increases the Efficiency of DNA-Mediated Transformation of Neurospora Crassa - http://www.fgsc.net/fgn38/selitren.html

Category: Fungi Protocols: Neurospora Protocols

Lipofectin increases the efficiency of DNA-mediated transformation of Neurospora crassa. - [Read Lipofectin Increases the Efficiency of DNA-Mediated Transformation of Neurospora Crassa]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]

Northern blot Protocol for the Detection of RNA in Neurospora - http://www.fgsc.net/neurosporaprotocols/Northern%20edited%20version.pdf

Category: Fungi Protocols: Neurospora Protocols

Northern blot protocol for the detection of RNA in Neurospora. Includes: Extract RNA from tissue powder; Electropheresis and transfer RNA; Preparing the membrane for probing and preparation of a riboprob.; Hybridization. - [Read Northern blot Protocol for the Detection of RNA in Neurospora]

Preparing and Transforming Spheroplasts of Neurospora crassa Protocol - http://www.fgsc.net/neurosporaprotocols/How%20to%20Prepare%20Spheroplasts.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for preparing and transforming spheroplasts of Neurospora crassa. The procedure is designed to produce a large number of spheroplasts to be stored frozen at -80°. - [Read Preparing and Transforming Spheroplasts of Neurospora crassa Protocol]

Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol - http://www.fgsc.net/neurosporaprotocols/VM%20vacuole%20procedure.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa (4 liter prep, easily scaled up to 10, 12, 24, or 30 l). - [Read Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol]

Small Scale DNA Preps for Neurospora crassa Protocol - http://www.fgsc.net/fgn/irelan.html

Category: Fungi Protocols: Neurospora Protocols

DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR. Starting with fresh mycelium, 20 to 40 samples can be processed in approximately two hours. Better yields (about 5 micrograms) may be obtained by suspending approximately 100 microliters of ground lyophilized mycelium in 500 microliters of isolation buffer and following the protocol. - [Read Small Scale DNA Preps for Neurospora crassa Protocol]

Toxicity of Antibiotics and Other Drugs to Neurospora - http://www.fgsc.net/methods/fgn21tox.html

Category: Fungi Protocols: Neurospora Protocols

Information and tips on the toxicity of antibiotics and other drugs to Neurospora. - [Read Toxicity of Antibiotics and Other Drugs to Neurospora]

Ultra-Fast Method of DNA Extraction from Neurospora Protocol - http://www.fgsc.net/fgn/camb.html

Category: Fungi Protocols: Neurospora Protocols

Simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation. - [Read Ultra-Fast Method of DNA Extraction from Neurospora Protocol]