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Calcium Phosphate Transfection of Neurons in Primary Culture - http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Ca_P_TX_R.shtml

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Detailed Neuronal cell transfection protocol and methods. Steven Finkbeiner. UCSF. - [Read Calcium Phosphate Transfection of Neurons in Primary Culture]

Combined Patch Clamp Recording and mRNA Expression Profiling in Individual Neurons - http://stokes.chop.edu/web/coulter/techniques.php

Category: Neuroscience Protocols: Patch Clamping Protocols

Combined patch clamp recording and mRNA expression profiling in individual neurons. - [Read Combined Patch Clamp Recording and mRNA Expression Profiling in Individual Neurons]

Culture of Dissociated Cortical Neurons Protocol - http://crutcherlab.med.uc.edu/DCN.htm

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol for culture of dissociated cortical neurons. - [Read Culture of Dissociated Cortical Neurons Protocol]

Culture of Dissociated Sympathetic Neurons Protocol - http://crutcherlab.med.uc.edu/DSN.htm

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol for culture of dissociated sympathetic neurons. - [Read Culture of Dissociated Sympathetic Neurons Protocol]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=14

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for the development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=15

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. Includes: Optimized cell culture protocol. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=16

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part]

Differentiate ES cells into glial cells and neurons - http://www.mshri.on.ca/nagy/Protocols/neurdif.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Differentiate ES cells into glial cells and neurons. Nagy Lab. - [Read Differentiate ES cells into glial cells and neurons]

Dye Filling to Stain Amphid and Phasmid Neurons Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=9

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol]

Dye Filling to Stain Amphid and Phasmid Neurons Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=9

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol]

Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol - http://www.cshprotocols.org/cgi/content/extract/2007/3/pdb.ip28

Category: Cytoskeleton Protocols: Actin Myosin Protocols

The same GFP-tagged actin construct used in cell transfection experiments has been used to produce transgenic mice. Transgenic animals allow the imaging of brain tissue in the intact animal, as acutely cut slices or as organotypic slice cultures. They also serve as a source of cells for imaging neurons at high resolution in dispersed low-density cell culture. In contrast to cells transfected in culture, where the level of actin-GFP expression in neurons varies considerably, transgenic mice... - [Read Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol]

Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death - http://neuroscience.jhu.edu/martin9-23.pdf

Category: Neuroscience: Neuron Cell Culture Protocols

Neuronal Cell Culture PDF. Lesuisse and Martin. Wiley Periodicals 2002. - [Read Long-Term Culture of Mouse Cortical Neurons as a Model for Neuronal Development, Aging, and Death]

Mechanical Tracing of Protein Function in the Drosophila Ear Protocol - http://www.natureprotocols.com/2006/11/03/mechanical_tracing_of_protein.php

Category: Drosophila Protocols

Method relies on the examination of the ear’s mechanics, which is actively modulated by the motility of auditory neurons and reflects the function of mechanosensory proteins these cells comprise [5-7]. Mechanical signatures arising from the motility of the neurons are assayed by measuring the vibrations of the antennal sound receiver in the presence and absence of sound. - [Read Mechanical Tracing of Protein Function in the Drosophila Ear Protocol]

Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation - http://www.natureprotocols.com/2006/09/26/neuronfibroblast_coculture_ass.php

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation. - [Read Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation]

Patch Clamp Measurements Applied to Neurons - http://www.4colorvision.com/neuron/patchclamp.htm

Category: Neuroscience Protocols: Patch Clamping Protocols

Patch clamp measurements applied to neurons. - [Read Patch Clamp Measurements Applied to Neurons]

Rat Neuron Nucleofector Kit - http://www.amaxa.com/fileadmin/groups/marketing/Downloads/Protocols/Primary_cells/amaxa_OP_Neuron_hippo_cortical_rat_DPG-1003.pdf

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Rat Neuronal Cell Culture Protocols

Optimized Protocol for primary rat hippocampal or cortical neurons. - [Read Rat Neuron Nucleofector Kit]

Staining of Tissue Slices for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4507

Category: RNAi Technology Protocols: RNAi Avian Embryos Protocols

Protocol describes a method to stain nerve fibers in tissue slices of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. The tissue is cut in slices using a vibratome or tissue slicer. The protocol is suitable for older embryos after approximately stage 33 and regions that are not accessible by whole-mount analysis. - [Read Staining of Tissue Slices for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos]

Staining of Tissue Slices for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4507

Category: Avian Bird Protocols

Techniques for Primary Culture of C. elegans Embryonic Neurons - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=14

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons]

Techniques for Primary Culture of C. elegans Embryonic Neurons II - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=15

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C.elegans embryonic neurons. - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons II]

Techniques for Primary Culture of C. elegans Embryonic Neurons III - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=16

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: C. elegans Neuronal Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons - [Read Techniques for Primary Culture of C. elegans Embryonic Neurons III]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol]

Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4445

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes transfection of plasmid DNA into primary hippocampal neurons using DNA/calcium-phosphate (CaPO4) coprecipitation. The precise pH of the transfection medium and the incubation time of cells with the coprecipitate are critical for reproducible and efficient transfection. Once these parameters are optimized for a given plasmid, the method is easily adapted for transfection of other established cell lines. - [Read Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation]

Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4506

Category: RNAi Technology Protocols: RNAi Avian Embryos Protocols

Protocol describes a method for staining nerve fibers in whole-mount preparations of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. This protocol has been successfully applied for embryos at different stages up to about stage 33 (7 days of incubation). - [Read Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos]

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