needles Protocols

Search results for: needles

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4417

Category: Stem Cell Protocols: Stem Cell Injection Protocols

Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips. - [Read Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol]

How to Make Tapered Platinum-Iridium Needles and Use Them for Isolating Ascospores - http://www.fgsc.net/neurosporaprotocols/How%20to%20make%20tools%20for%20spore%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to make tapered platinum-iridium needles and use them for isolating ascospores. - [Read How to Make Tapered Platinum-Iridium Needles and Use Them for Isolating Ascospores]

Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4651

Category: Microinjection Protocols: Microinjection Equipment

This protocol contains methods for pulling microinjection needles using two different models of pipette pullers. The advantage of pulling needles in the laboratory is that a variety of different needle types can be pulled, depending on the samples and cells being injected. An added advantage is cost; once a pipette puller has been purchased, boxes of glass capillaries are inexpensive compared to premade microinjection needles. - [Read Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol]

Purification of RNA from Plant Tissue Using the Concert Plant Reagent - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4106

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

This protocol is not phenol-based, but does require the addition of chloroform. The Concert Plant Reagent is intended for the isolation of RNA from a wide variety of plant tissues including blue spruce needles, potato tuber etc. - [Read Purification of RNA from Plant Tissue Using the Concert Plant Reagent]

RNA Extraction and cDNA from Pine Needles - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb21/r03-031.pdf

Category: RNA Protocols: RT-PCR and cDNA synthesis

RNA Extraction and cDNA from Pine Tree Needles - [Read RNA Extraction and cDNA from Pine Needles]