needle Protocols

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Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4417

Category: Stem Cell Protocols: Stem Cell Injection Protocols

Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips. - [Read Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol]

DNA Preparation and Needle Loading for Gene Delivery by Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4652

Category: Microinjection Protocols: Microinjection of DNA Protocols

For microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach. - [Read DNA Preparation and Needle Loading for Gene Delivery by Microinjection Protocol]

Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4653

Category: Microinjection Protocols: Microinjection of DNA Protocols

This protocol describes a method for pulsed-flow microinjection using the Eppendorf FemtoJet injector and Eppendorf InjectMan; this is the most common type of pulsed-flow microinjection system currently being used. The advantage of this type of system over a controlled-flow system is that much more control is available over the injection parameters, reducing variability in injections. In addition, the system allows a diagonal insertion of the needle into the cell. - [Read Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol]

Making a Tetrad Dissection Needle Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4182

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Microneedles attached to micromanipulators are used in the dissection of tetrads, isolation of zygotes from populations of mating haploid cells, and manipulation of individual cells. - [Read Making a Tetrad Dissection Needle Protocol]

Microinjecting C.elegans Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=5

Category: C. elegans Protocols: C. elegans Injection Protocols

Protocol for microingecting C.elegans worms. Includes: Making the DNA solution; Setting up the scope, loading the needle, mounting, and breaking the needle; Mounting worms on an injection pad; Injecting. - [Read Microinjecting C.elegans Worms Protocol]

Needle Assay for Chemotaxis - http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm

Category: Immunology: Chemotaxis

Needle Assay for Chemotaxis. Devreotes Lab, John Hopkins Medical Centre. - [Read Needle Assay for Chemotaxis]

Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4651

Category: Microinjection Protocols: Microinjection Equipment

This protocol contains methods for pulling microinjection needles using two different models of pipette pullers. The advantage of pulling needles in the laboratory is that a variety of different needle types can be pulled, depending on the samples and cells being injected. An added advantage is cost; once a pipette puller has been purchased, boxes of glass capillaries are inexpensive compared to premade microinjection needles. - [Read Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol]

Preparing Cell Smears from Tissue Samples for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4330

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Staining of tissues can be carried out on cell smears prepared from biopsy samples, such as needle aspirates, tissue scrapings, or freshly dissected tissues. - [Read Preparing Cell Smears from Tissue Samples for Immunostaining Protocol]

Protocol for Needle Assay for Chemotaxis - http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm

Category: Cell Biology and Cell Culture

Protocol for needle assay for chemotaxis. Materials required: Zeiss inverted microscope, eppendolf microinjector, eppendolf micromanipulator, Eppendolf femtotips, 100mM cAMP in PM,DB buffer, PM Buffer. - [Read Protocol for Needle Assay for Chemotaxis]