myocyte Protocols

Category: Signalling Signal Transduction Protocols

Protocol provides a method for acheiving a sufficient sample for several determinations of cAMP. The protocol described for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in cardiac myocytes is an enzyme-linked immunoassay system. Protocol includes information on: Treatment of Cells and Preparation of Extracts; Use of Environmental Chamber; Reagents and Materials. - [Read Preparation of Myocyte Lysates for Cyclic AMP Determination]

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol permits the isolation of at least 3 µg of total RNA from approximately 150,000 cultured cardiac myocytes from adult mice. Includes: Treatment of Samples and Termination of Reactions; Isolation of RNA; Use of Environmental Chamber. - [Read Preparation of Myocyte RNA for Microarray Analysis Protocol]