mycoplasmas Protocols

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Concentration of Oligo DNA by Ethanol Precipitation Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html

Category: Oligonucleotide Protocols: Oligonucleotide Purification Protocols

Concentration of DNA by Ethanol Precipitation Protocol. Adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma. Usually 2.5 - 3 volumes of ethanol and/or acetate solution is added to the DNA in a microcentrifuge tube. This is then put into an ice-water bath for at least 10 minutes. The precipitation is performed by incubation at -20C overnight. - [Read Concentration of Oligo DNA by Ethanol Precipitation Protocol]

ELISA Method - http://mycoplasmas.vm.iastate.edu/lab_site/methods/immunol/ELISA.html

Category: ELISA Protocols

Reagents, protocol and Conjugate Preparation. Minion Lab, College of Veterinary Medicine, Iowa State. - [Read ELISA Method]

Glass Milk Agarose Gel DNA Extracton Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/glass_milk.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Isolation of DNA fragments using glass milk (GENE-CLEAN). Glass Milk Agarose Gel DNA Extracton Protocol. Minion Lab, College of Veterinary Medicine at Iowa State University. - [Read Glass Milk Agarose Gel DNA Extracton Protocol]

Methods for DNA isolation - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/DNAisol.html

Category: DNA Protocols: DNA Extraction Protocols

Large scale double-stranded DNA isolation, Miniprep double-stranded DNA isolation, Large scale M13RF isolation, Single-stranded M13 DNA isolation using phenol. Minion Lab.Veterinary Medicine Iowa Univ. - [Read Methods for DNA isolation]

Mycoplasma Elimination Reagent Protocol - http://www.biovalley.fr/anglais/biology/mynang.htm

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

For both biological and economical reasons, it is important to eliminate mycoplasmas from cell cultures being used for basic research, diagnosis, and biotechnological production. The most commonly used method for elimination, inactivation, or suppression of mycoplasmas in cell cultures is treatment with antibiotics. In general, antibiotic therapies do not result in long-lasting, successful elimination. Also, the cytotoxic properties of antibiotics can cause undesirable side effects on cells. - [Read Mycoplasma Elimination Reagent Protocol]

SDS-PAGE Gels - http://mycoplasmas.vm.iastate.edu/lab_site/methods/protein/SDS-PAGE.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE Gels. Adapted from David Bowtell - [Read SDS-PAGE Gels]