User Panel

My Panel

Bookmark Science Articles

mutants Protocols

Search results for: mutants

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Aspergillus nidulans Mutants - http://www.fgsc.net/alist.html

Category: Fungi Protocols: Aspergillus Protocols

Compilation of A. nidulans mutants listing names, affected enzymes (or gene functions), linkage assignments, properties of the mutants, and names of corresponding loci in N. crassa. - [Read Aspergillus nidulans Mutants]

Forward Genetics in Arabidopsis: Finding Mutations that Cause Particular Phenotypes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/26/pdb.top1

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Forward genetics is used to identify genes that are involved in particular biological processes. For example, genes required for disease resistance can be found by identifying mutants with reduced or increased disease resistance, genes that control flower development can be identified by searching for mutants with altered flower morphology, and genes encoding enzymes for tryptophan biosynthesis can be identified by searching for mutants that require exogenous tryptophan for growth. - [Read Forward Genetics in Arabidopsis: Finding Mutations that Cause Particular Phenotypes Protocol]

Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols - http://www.fgsc.net/fgn48/Kaminskyj.htm

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3589

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

In this method, the nuclease BAL 31 is used to make uni- or bidirectional deletions in a segment of cloned DNA. BAL 31 is a complex enzyme and tends to digest a population of double-stranded DNA targets in an asynchronous fashion, Deletions created by BAL 31 are therefore far more heterogeneous in size than those created by processive enzymes such as exonuclease III. - [Read Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease Protocol]

Generation of Sets of Nested Deletion Mutants with Exonuclease III Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4078

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

The double-stranded DNA of recombinant plasmid, phagemid, or bacteriophage M13 replicative form DNA is digested with two restriction enzymes whose sites of cleavage both lie between one end of the target DNA and the binding site for universal primer. The enzyme that cleaves nearer the target sequence must generate either a blunt end or a recessed 3' terminus; the other enzyme must generate a four-nucleotide protruding 3' terminus. - [Read Generation of Sets of Nested Deletion Mutants with Exonuclease III Protocol]

How to Use Asci for Obtaining Double Mutants - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20asci%20for%20obtaining%20double%20mutants%20of%20genes%20that%20show%20epistasis%20of%20are%20phenotypically%20similar.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use asci for obtaining double mutants of genes that show epistasis or are phenotypically similar. - [Read How to Use Asci for Obtaining Double Mutants]

How to Use Helper Strains for Maintaining and Crossing Handicapped Recessive Mutants - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20helper%20strains%20for%20maintaining%20and%20crossing%20handicapped%20recessive%20mutants,%20for%20forcing%20and%20resolving%20heterokaryons,%20and%20for%20determining%20heterokaryon%20compatibility

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use helper strains for maintaining and crossing handicapped recessive mutants, for forcing and resolving heterokaryons, and for determining heterokaryon compatibility. - [Read How to Use Helper Strains for Maintaining and Crossing Handicapped Recessive Mutants]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www-rcf.usc.edu/~forsburg/hydroxyl.html

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for hydroxylamine mutagenesis of plasmid DNA is ideal for random mutagenesis of plasmid DNA which is then used in a plasmid shuffle or screen for ts mutants. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA. - [Read In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4173

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

It is sometimes desirable to have an entire population of yeast cells that is growing synchronously or is arrested at a unique point in the cell cycle. - [Read Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol]

Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3284

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Protocol used chiefly to generate large stocks of double-stranded DNA of strains of M13 that are routinely used as cloning vectors. Large amounts of single-stranded DNA of an individual recombinant may occasionally be needed for specific purposes, e.g., to generate many preparations of a particular radiolabeled probe or to construct large numbers of site-directed mutants. - [Read Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol]

Mapping C. elegans Mutants using STS Polymorphisms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=32

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for mapping C. elegans mutants using STS polymorphisms. - [Read Mapping C. elegans Mutants using STS Polymorphisms Protocol]

Protocol Live Imaging of Caenorhabditis Elegans - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip19

Category: Microscopy Protocols: In Vivo Imaging Protocols

To image early cleavages and chromatin dynamics, it is convenient to use histone H2B fused to GFP or lamin::GFP. Time-lapse movies can be obtained using conventional confocal microscope systems and their included software. Early embryos dissected from transgenic hermaphrodites are placed with egg salts on agar pads. Chromatin dynamics can be followed easily, and wild-type embryonic cells can be compared with mutants or RNAi-treated embryos. - [Read Protocol Live Imaging of Caenorhabditis Elegans]

Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./rapds.html~main

Category: Fungi Protocols: Aspergillus Protocols

RAPD is a procedure for typing and fingerprinting isolates of a species. It can be used for epidemiological studies, such as investigations into hospital outbreaks and as a laboratory aid to keep track of cultures and to verify that mutants generated in the laboratory are genetically identical to the parental strain. In our hands, the use of one primer, R108, is sufficiently discriminatory to distinguish between the isolates of different strains. - [Read Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol]

Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/missegscreen.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for a rapid screen for chromosome missegregation mutants by DAPI staining. - [Read Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol]

Root Transformation of Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4671

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Arabidopsis can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. We describe the generation of transgenic plants via root transformation in tissue culture, which can be useful for transforming sterile mutants. - [Read Root Transformation of Arabidopsis Protocol]

Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol - http://www.genome.wisc.edu/functional/tnmutagenesis.htm

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for systematic mutagenesis of E.coli K-12 MG1655 ORFs. Includes: Mutagenesis Project Progress; Construction of Sce-poson mutants; Mutagenesis of Essential Genes; Gene Gorging. - [Read Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol]

Telomere Length Determination Protocol - http://www-rcf.usc.edu/~forsburg/telomere.html

Category: Cell Biology and Cell Culture

Telomere length can vary from strain to strain and is sensitive to a variety of mutants affecting DNA and chromosome function. Includes: Gel Protocol; Southern blot using oligo probe. - [Read Telomere Length Determination Protocol]

Articles (1-1 of 1)

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.