User Panel

My Panel

Bookmark Science Articles

mutagenesis Protocols

Search results for: mutagenesis

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A Targeted Screen to Detect Recessive Mutations that have Quantitative Effects Protocol - http://www.nervenet.org/papers/Consomic.html

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

Describes an experimental cross in mice that can be used to define and map induced germ-line mutations that map to a single chromosome. The cross is a modification and extension of a conventional three-generation recessive mutagenesis screen. Includes: The Mutagenesis Breeding Plan; Consomic Strains; Generating Mutations; Generating and Genotyping G2 Females; Genotyping G3 Progeny; Phenotyping G4 Progeny; etc.. - [Read A Targeted Screen to Detect Recessive Mutations that have Quantitative Effects Protocol]

C. elegans EMS Mutagenesis Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=26

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans EMS mutagenesis. - [Read C. elegans EMS Mutagenesis Protocol]

Creating a Deletion by PCR Splicing Protocol - http://www.methods.info/Tips/mutagenesis/PCR_splicing.html

Category: Cloning Protocols: Mutagenesis Protocols

This approach can be used to delete a fragment of any lenth or to introduce point mutations into a DNA seqence. - [Read Creating a Deletion by PCR Splicing Protocol]

EMS Mutagenesis of Arabidopsis Seed Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4621

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

EMS is used at concentrations that induce multiple point mutations in each plant, such that mutant alleles of a specific locus are found at a rate of ~1 in 2000-5000 M2 plants. This high rate of mutagenesis makes possible the screening of relatively few plants to find those with the phenotype of interest, a particular advantage if the screen is laborious or if only a small number of genes mutate to the required phenotype. - [Read EMS Mutagenesis of Arabidopsis Seed Protocol]

EMS Mutagenesis of C. elegans Protocol - http://www.k-state.edu/hermanlab/protocols/ems_mutagenesis.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for EMS mutagenesis of C. elegans. - [Read EMS Mutagenesis of C. elegans Protocol]

EMS Mutagenesis of Yeast Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/ems_mutagenesis.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for EMS mutagenesis of yeast. - [Read EMS Mutagenesis of Yeast Protocol]

ENU Mutagenesis Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/enu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for ENU mutagenesis. - [Read ENU Mutagenesis Protocol]

Ethyl Methane Sulfonate (EMS) Mutagenesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4180

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol describes mutagenesis of yeast with ethyl methane sulfonate (EMS). It causes approximately 40-70% cell death in most haploid laboratory strains, a level of cell killing that is commonly used in mutant hunts with haploid strains. - [Read Ethyl Methane Sulfonate (EMS) Mutagenesis Protocol]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

How to Use Chemical Mutagens for Mutagenesis - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20chemical%20mutagenesis.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use chemical mutagens for mutagenesis. - [Read How to Use Chemical Mutagens for Mutagenesis]

How to Use UV for Mutagenesis - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20UV%20for%20mutagenesis.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use UV for mutagenesis. - [Read How to Use UV for Mutagenesis]

Hydoxylamine Mutagenesis of Plamid DNA Protocol - http://rothlab.ucdavis.edu/protocols/HA-mutagenesis.html

Category: Bacteria Genetics Protocols

Protocol for hydoxylamine mutagenesis of plasmid DNA. - [Read Hydoxylamine Mutagenesis of Plamid DNA Protocol]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4156

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol produces mutagenized DNA that can be used for transformation of either Escherichia coli or yeast. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www-rcf.usc.edu/~forsburg/hydroxyl.html

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for hydroxylamine mutagenesis of plasmid DNA is ideal for random mutagenesis of plasmid DNA which is then used in a plasmid shuffle or screen for ts mutants. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA. - [Read In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI]

Molecular and Genetic Toxicology Studies on Microbial Mutagenesis - http://www.sri.com/biosciences/bdd/toxic/protocol.html#4

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies on microbial mutagenesis. Includes: G101 Salmonella/Microsome Plate Incorporation Assay (Ames Test), G101-R, G102 Salmonella/Microsome Preincubation Assay (Ames Test), G102-R, G103 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and one E. coli strains), G104 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and two E. coli strains), G105, G106. - [Read Molecular and Genetic Toxicology Studies on Microbial Mutagenesis]

Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis) - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3742

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for oligonucleotide-directed mutagenesis by elimination of a unique restriction site (USE mutagenesis). - [Read Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis)]

Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3817

Category: Cloning Protocols: Mutagenesis Protocols

Protocool for oligonucleotide-directed mutagenesis of single-stranded DNA. - [Read Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol]

Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3811

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Single-stranded templates of bacteriophage M13 DNA containing 20-30 residues of uracil in place of thymine are generated during growth of the bacteriophage in an F' strain of E. coli carrying mutations in the ung and dut genes. This DNA is used as a template in the Kunkel method of oligonucleotide-directed mutagenesis (Oligonucleotide-directed Mutagenesis of Single-stranded DNA). - [Read Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol]

Pristionchus Pacificus Genetic Protocols - http://www.wormbook.org/chapters/www_ppageneticprotocols/ppageneticprotocols.pdf

Category: Developmental Biology: Utilizing Model Organisms Protocols

Protocols on the genetics of Pristionchus pacificus. Includes: Freezing worms; Mutagenesis; Construction of deletion libraries to generate P. pacificus gene knock-outs; RNAi and morpholino by injection. - [Read Pristionchus Pacificus Genetic Protocols]

Pristionchus pacificus Genetic Protocols - http://www.wormbook.org/chapters/www_ppageneticprotocols/ppageneticprotocols.pdf

Category: Laboratory Model Organisms Protocols

Genetic Protocols for Pristionchus pacificus. Includes: Freezing worms; EMS mutagenesis; Psoralen mutagenesis; Construction of deletion libraries to generate P. pacificus gene knock-outs; Designing primers for the gene of interest; RNAi and morpholino by injection. - [Read Pristionchus pacificus Genetic Protocols]

Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3467

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for rapid and efficient site-directed mutagenesis by the single-tube megaprimer PCR method. - [Read Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol]

Rapid PCR Site-Directed Mutagenesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4144

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for rapid PCR site-directed mutagenesis. - [Read Rapid PCR Site-Directed Mutagenesis Protocol]

Screening Recombinant Clones for Site-directed Mutagenesis by Hybridization to Radiolabeled Oligos - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4013

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

Plaques formed by M13 bacteriophages or bacterial colonies transformed by plasmids carrying specific mutations can be detected by hybridization, using a radiolabeled oligonucleotide that forms a perfect duplex with the mutant sequence. Hybridization is carried out under conditions of low stringency that allow the radiolabeled oligonucleotide to anneal to both mutant and wild-type DNAs. - [Read Screening Recombinant Clones for Site-directed Mutagenesis by Hybridization to Radiolabeled Oligos]

Site Directed Mutagenesis Protocol - http://openwetware.org/wiki/Knight:Site-directed_mutagenesis

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for site directed mutagenesis. - [Read Site Directed Mutagenesis Protocol]

Articles (1-1 of 1)

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Items 1-25 out of 32 displayed.

First [1] 2 Next Last

Total records: 32