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Cap-Switching RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4133

Category: PCR Protocols: cDNA Synthesis Protocols

This method, for the selective amplification of full-length cDNA ends, involves the addition of an adapter during reverse transcription. This method takes advantage of the propensity of Moloney murine leukemia virus reverse transcriptase (MMLV RT) to append two to four cytosines to the 3'-end of newly synthesized cDNA strands. The additional residues are added when the enzyme reaches the 5'-cap structure at the end of the mRNA template. - [Read Cap-Switching RACE Protocol]

Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Describes the standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells (HSC) and Myeloid Progenitors]

Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA - http://www.natureprotocols.com/2006/06/29/cell_staining_for_sorting_of_h.php

Category: Stem Cell Protocols

The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. Here, we described our standard procedures of the murine myeloid progenitor staining for fluorescence activated cells sorting (FACS) and RNA purification methods. - [Read Cell Staining for Sorting of Hematopoietic Stem Cells and Myeloid Progenitors and Isolating RNA]

Immunofluorescent Staining for Flow Cytometry Protocols - http://www.ebioscience.com/ebioscience/appls/FCS.htm

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocols for immunofluorescent staining for flow cytometry. Includes: Protocol for Staining of Cell Suspensions of Murine Lymphoid Tissue; Protocol for Staining of Human Peripheral Blood; Fluorescent Dyes for Flow Cytometric Analysis - [Read Immunofluorescent Staining for Flow Cytometry Protocols]

Isolation of Murine Macrophages Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6632549C959D0FFF7FD119F10D62D9&objectid=667572BADF718B4599BE6AE4ADD48CB3

Category: Mouse Protocols

Protocol that investigates macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3. - [Read Isolation of Murine Macrophages Protocol]

Murine Osteoblast Harvest Protocol - http://www.labprotocol.com/detail.php?siteid=32

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for murine osteoblast harvest. - [Read Murine Osteoblast Harvest Protocol]

Osteoblast Harvest (murine) Protocol - http://www.labprotocol.com/detail.php?siteid=32

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol for osteoblast harvest (murine). - [Read Osteoblast Harvest (murine) Protocol]

Preparation of NeoR Murine Embryonic Fibroblasts Protocol - http://ourworld.compuserve.com/homepages/TheBroons/tnp7.htm

Category: Stem Cell Protocols: Feeder Cell Preparation Protocols

Protocol for the preparation of NeoR murine embryonic fibroblast. - [Read Preparation of NeoR Murine Embryonic Fibroblasts Protocol]

Preparation of NeoR Murine Embryonic Fibroblasts Protocol - http://ourworld.compuserve.com/homepages/TheBroons/tnp7.htm

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Protocol for the preparation of NeoR murine embryonic fibroblasts. - [Read Preparation of NeoR Murine Embryonic Fibroblasts Protocol]

Preparation of NeoR Murine Embryonic Fibroblasts Protocol - http://ourworld.compuserve.com/homepages/TheBroons/tnp7.htm

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for the preparation of NeoR murine embryonic fibroblasts. - [Read Preparation of NeoR Murine Embryonic Fibroblasts Protocol]

Production of T Cell Clones Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66347791E1CAD019BE41F3277340CA&objectid=6674BCB8F6BCF7B8032EEBAF7A3D08BD

Category: Immunology: T Cell Protocols

Provides methods for the derivation of specific types of T cell clones: preparation and maintenance of alloreactive murine helper T (TH) lymphocyte and cytotoxic T lymphocyte (CTL) clones using the limiting dilution technique and derivation of TH clones reactive with soluble protein antigens including a method for the selection of either TH1 or TH2 lymphocyte subsets. - [Read Production of T Cell Clones Protocol]

Quantitation of Functional T Cells by Limiting Dilution Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6634A6F151B8D376E18500D58E4C11&objectid=6674C301AD333544C89D384F6FCE439A

Category: Immunology: T Cell Protocols

In the first protocol, IL-2-producing murine T cells are measured following stimulation by the mitogen Con A. The second protocol provides a modification for using human responder cells. The second protocol is used for estimating the proportion of cells that can generate a clone of cytotoxic effector cells when stimulated by Con A with the addition of IL-2. - [Read Quantitation of Functional T Cells by Limiting Dilution Protocols]

Transgenic - Universal Mouse Genotyping Protocol Using PCR - http://www.knockoutmousestation.com/mouse/detail/link-28.html

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Description:, Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. ... - [Read Transgenic - Universal Mouse Genotyping Protocol Using PCR]

Universal Mouse Genotyping Protocol - http://ddrcc.wustl.edu/Genotyping_Protocol.html

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Universal Mouse Genotyping Protocol (downloadable pdf click here). This protocol is designed to detect sequences in the murine genome by polymerase chain ... - [Read Universal Mouse Genotyping Protocol]

Universal Mouse Genotyping Protocol - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

This protocol is designed to detect sequences in the murine genome by polymerase chain reaction amplification, and is adapted from Stratman and Simon (Transgenic Res. 12, 521-522 (2003)).For those familiar with PCR genotyping, this method differs from the typical protocol by utilizing a unique enzyme (Klentaq), 30mer primers, and a 68° annealing temperature. - [Read Universal Mouse Genotyping Protocol]

Universal Mouse Genotyping Protocol Using PCR - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. - [Read Universal Mouse Genotyping Protocol Using PCR]

Articles (1-1 of 1)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.