multiwell Protocols

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96-Well Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/96_well_confirmation.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Cell Viability Information For Protocols and Applications - http://www.promega.com/paguide/chap4.htm

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Includes information on: Establishing an In Vitro Model System; Choosing an Endpoint to Measure; Characterizing Assay Responsiveness; Determining Dose and Duration of Exposure; Homogeneous Assays for Multiwell Formats and Automated Screening; Additional Factors to Consider When Choosing a Cell Viability Assay; Cell Viability Assays that Measure ATP Protocol; etc.. - [Read Cell Viability Information For Protocols and Applications]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Multiwell Transformation Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/transprot.html

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for multiwell transformation. - [Read Multiwell Transformation Protocol]

SNP Genotyping System - http://www.promega.com/tbs/tm053/tm053.pdf

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]