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Universal Mouse Genotyping Protocol - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

This protocol is designed to detect sequences in the murine genome by polymerase chain reaction amplification, and is adapted from Stratman and Simon (Transgenic Res. 12, 521-522 (2003)).For those familiar with PCR genotyping, this method differs from the typical protocol by utilizing a unique enzyme (Klentaq), 30mer primers, and a 68° annealing temperature. - [Read Universal Mouse Genotyping Protocol]

Universal Mouse Genotyping Protocol Using PCR - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. - [Read Universal Mouse Genotyping Protocol Using PCR]

Uterine Transfer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4380

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a procedure for uterine transfer, which is used for chimera production. The method is based on extensive work which resulted in the first successful development and birth of in-vitro-cultured mouse embryos. It is best to practice this procedure first on a cadaver and then on an anesthetized 2.5-days post coitum (dpc) pseudopregnant mouse using blue Affigel beads rather than embryos. - [Read Uterine Transfer Protocol]

Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4377

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a method for vasectomy in which the vas deferens is accessed through the scrotal sac. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. - [Read Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols]

Vasectomy of Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp9.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for the vasectomy of mouse. Includes: The Anesthetic; Test Breeding. - [Read Vasectomy of Mouse Protocol]

Vincenzo Covelli - Guide to the Necropsy of the Mouse - http://www.eulep.org/Necropsy_of_the_Mouse/index.php

Category: Animal Research Techniques

Vincenzo Covelli - Guide to the Necropsy of the Mouse. Rome Italy - [Read Vincenzo Covelli - Guide to the Necropsy of the Mouse]

Whole Mount In Situ Hybridization of Mouse Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/in_situ.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization. - [Read Whole Mount In Situ Hybridization of Mouse Protocol]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

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