motility Protocols

Search results for: motility

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC Protocol - http://mitchison.med.harvard.edu/protocols/flowcell.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for flow cell assays with microtubules. Includes: Solutions & Supplies; VE-DIC Assays; Motility; Dynamics; Fluorescence Assays; Oxygen Scavenging; Assays. - [Read Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC Protocol]

Mechanical Tracing of Protein Function in the Drosophila Ear Protocol - http://www.natureprotocols.com/2006/11/03/mechanical_tracing_of_protein.php

Category: Drosophila Protocols

Method relies on the examination of the ear’s mechanics, which is actively modulated by the motility of auditory neurons and reflects the function of mechanosensory proteins these cells comprise [5-7]. Mechanical signatures arising from the motility of the neurons are assayed by measuring the vibrations of the antennal sound receiver in the presence and absence of sound. - [Read Mechanical Tracing of Protein Function in the Drosophila Ear Protocol]

Microtubule/ organelle Motility Assays - http://www.bio.unc.edu/faculty/salmon/lab/protocolsmtorganellemotilityassays.html

Category: Cell Biology and Cell Culture

Microtuble/ organelled motility assay using Golgi or ER membranes, 45 uM tubulin, rat liver cytosol, and 20x energy regeneration system. - [Read Microtubule/ organelle Motility Assays]

Protocol for Microtubule Assembly - http://www.bio.com/protocolstools/protocol.jhtml?id=p1600

Category: Cell Biology and Cell Culture

Purified Tubulin is polymerized and then stabilized with Taxol. The resulting Microtubules are then ready for use in motility assays. Protocol use solutions such as 50 mM Mg-GTP, 50 Î¼l 100 mM GTP, 40 Î¼M Taxol, Taxol Stock,and PM Buffer. - [Read Protocol for Microtubule Assembly]

Protocol for Motility using VE-DIC Microscopy - http://www.proweb.org/kinesin/Methods/VE-DIC.html

Category: Cell Biology and Cell Culture

Protocol for motility using VE-DIC microscopy. Materials required: Anti-GST (glutathione S-transferase) antibody, PB buffer =10 mM NaPO4 pH 7.2, EGTA, MgCl2, Clarified motor lysate, MTs or Axoneme-MTs,MgÂ·ATP, VALAP (1 Vasoline: 1 Lanolin: 1 Paraffin, heated gently until melted) and Maxell XR-S120 Black Magnetite Super-VHS tapes or comparable. - [Read Protocol for Motility using VE-DIC Microscopy]

Protocol for Standard Fluorescence Motility Assay - http://www.proweb.org/kinesin/Methods/motility.html

Category: Cell Biology and Cell Culture

Protocol for Standard Fluorescence Motility Assay. Materials requires: Microtubules, Flow Cell, Standard Solutions: BRB80, BRB80T, BRB80CS0.5, BRB80CA, BRB80CT - [Read Protocol for Standard Fluorescence Motility Assay]

Tetrahymena Thermophila Ocular Irritancy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/22.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with the normal motility of the protozoan, Tetrahymena thermophila, in culture. The degree of interference of motility as compared to control cultures, related to the concentration of the test compound, provides an indication of toxicity. - [Read Tetrahymena Thermophila Ocular Irritancy Test]