monocyte Protocols

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Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=572&criteria=

Category: Immunology: Mononuclear Cell Isolation Protocols

Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used in the form of L929 cell conditioned medium. The progenitor cells proliferate and differentiate through monoblast, promonocyte and monocyte stages before maturing to macrophages. At this time the cells become firmly adherent to the culture vessel. - [Read Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol]

Isolation of monocytes from monocyte enriched PBMC fraction using CD14 - http://www.methods.info/Methods/Cell_biology/Monocytes_positive.pdf

Category: Cell Biology and Cell Culture

This protocol uses the PBMC fraction enriched in with monocytes by density gradient centrifugations. Reduction of the amount of microbeads reduces the costs of the experiment. - [Read Isolation of monocytes from monocyte enriched PBMC fraction using CD14]

MONOCYTE AND NEUTROPHIL ISOLATION - http://aactg.s-3.com/pub/download/imm/phagocyt.pdf

Category: Immunology: Chemotaxis

MONOCYTE AND NEUTROPHIL ISOLATION. The protocol describes the method used for isolation of monocytes and neutrophils used in both the chemotaxis assay and microbicidal assays. Pediatric AIDS Clinical Trials Group - [Read MONOCYTE AND NEUTROPHIL ISOLATION]