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Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

Baculovirus Production for Recombinant Proteins - http://axon.med.harvard.edu/~cepko/protocol/mike/B1.html

Category: Protein Protocols: Protein Expression Protocols

Baculovirus Production for Recombinant Proteins. Protocol is a modified version of the pharmingen Baculogold kit. Brian Dynlacht. Mike A. Dyer Lab. - [Read Baculovirus Production for Recombinant Proteins]

Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3871

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

Calcium phosphate forms an insoluble precipitate with DNA, which attaches to the cell surface and is taken into the cells by endocytosis. The protocol is easily adapted for use with other types of cells, both adherent and nonadherent. This protocol is a modified version of a method published by Jordan et al. (1996) who rigorously optimized calcium-phosphate-based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells. - [Read Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs]

Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber) - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2158

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Simple apparatus to study chemotaxis of leukocytes or other migratory cells. Protocol for Chemotaxis. BioProtocol - [Read Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)]

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion - http://www.animal.ufl.edu/hansen/protocols/endocult.htm

Category: Cell Culture Protocols

Protocol used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.The medium used is called Pig MEM, which is a modified minimum essential medium supplemented with non-essential amino acids, vitamins, insulin and additional glucose. - [Read Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion]

DNA Microprep Isolation from Plants Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p9010

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the advantage of isolating DNA from plant material very rapidly. The procedure requires a table-top drill-press (mechanized homogenizer). - [Read DNA Microprep Isolation from Plants Protocol]

DNA Miniprep Isolation from Plants Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1079

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the advantage of speed and its use of inexpensive reagents. - [Read DNA Miniprep Isolation from Plants Protocol]

Fission Yeast Media Protocol - http://www-rcf.usc.edu/~forsburg/media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for fission yeast media. Includes: YES (yeast extract with supplements) for rich complete media; YSO, for modified rich media; EMM (Edinburgh minimal medium) for minimal selective media; stock solutions to make up minimal media; MB, a very stringent minimal for transformation; Sporulation media; ME (malt extract); SPAS; EMMGlut. - [Read Fission Yeast Media Protocol]

Frame Modified Neutral Red Uptake Cytotoxcity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/3.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon cells in culture is measured by cell viability (neutral red uptake) method. - [Read Frame Modified Neutral Red Uptake Cytotoxcity Test]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. - http://www.emory.edu/WILCOX/tunel.paraf.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections. Wilcox et al. Modified Protocol. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling using Paraffin Sections.]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. - http://www.emory.edu/WILCOX/tunel.frzn.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. Wilcox et al. Modified. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections.]

Iscove’s Modified Dulbecco’s Media (IMDM) Protocol - http://www.sigmaaldrich.com/img/assets/11020/IMDM.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for Iscove’s modified dulbecco’s media (IMDM). - [Read Iscove’s Modified Dulbecco’s Media (IMDM) Protocol]

Isolation of Plant Transcription Factors Using a Modified Yeast One-Hybrid System - http://www.plantmethods.com/content/2/1/3

Category: Plant Biology Protocols: Plant Genetics Protocols

The preparation of expressional cDNA libraries for use in the yeast two-hybrid system is quick and efficient when using the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3. This kit employs SMART technology for the amplification of full-length cDNAs, in combination with cloning using homologous recombination. - [Read Isolation of Plant Transcription Factors Using a Modified Yeast One-Hybrid System]

Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4149

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol (modified from Hoffman and Winston 1987) describes the purification of genomic DNA from yeast cells. - [Read Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol]

Modified Thioflavin for Senile Plaques Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MODTHIOF.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol for modified thioflavin for senile plaques amyloid. - [Read Modified Thioflavin for Senile Plaques Amyloid Protocol]

Modified Topo T/A cloning protocol - http://mullinslab.microbiol.washington.edu/protocols/other/TopoTACloning.doc

Category: DNA Protocols: TOPO TA Cloning

Modified Topo T/A cloning protocol. Mullins Lab. Univ. Washington. - [Read Modified Topo T/A cloning protocol]

Nucleofection and Adoptive Transfer of Primary Mouse T lymphocytes using Transient Transfection - http://www.natureprotocols.com/2006/10/02/nucleofection_and_adoptive_tra.php

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection of genes into naïve mouse T cells using nucleofection using a modified electroporation technique. This protocol offers the possibility for rapid functional in vivo studies of targeted genes in primary mouse T cells. - [Read Nucleofection and Adoptive Transfer of Primary Mouse T lymphocytes using Transient Transfection]

Quantification of DNA Methylation in Electrofluidics Chips Protocol - http://www.natureprotocols.com/2006/06/23/quantification_of_dna_methylat.php

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for quantification of DNA methylation in electrofluidics chips. Describe Bio-COBRA, a modified protocol for Combined Bisulfite Restriction Analysis (COBRA), that incorporates an electrophoresis step in microfluidics chips. Microfluidics technology involves the handling of small amounts of liquid in miniaturized systems. - [Read Quantification of DNA Methylation in Electrofluidics Chips Protocol]

Reagents and Guidelines for Long PCR - http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Long PCR Protocols

Long PCR Buffer, Cycle times and temperatures, Picking Primers, Hot Start Polymerases for Long PCR. Harvard-Lipper Center for Computational Genetics. Modified from Cheng et al. - [Read Reagents and Guidelines for Long PCR]

RNA Labeling by In Vitro Transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_54-57.pdf

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

Protocol for RNA labeling by in vitro transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix. A PCR fragment that has the appropriate promoter ligated to its 5’-ends can also serve as a transcription template. The procedure described incorporates one modified nucleotide (DIG-, Biotin-, or Fluorescein-UTP) at approximately every 20 – 25th position in the transcripts. - [Read RNA Labeling by In Vitro Transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix]

SDS-PAGE & Western Blotting Protocols - http://micro.mic.ucdavis.edu/singer/protocols/SDS-PAGEandWesternBlot.pdf

Category: Protein Protocols: Western Blot Protocol

Adapted from existing protocols by Vinh PhamLast modified: June 5, 2003. - [Read SDS-PAGE & Western Blotting Protocols]

Tandem Affinity Purification (TAP) Protocol - http://depts.washington.edu/~yeastrc/ms_tap1.htm

Category: Mass Spectrometry Protocols

Tandem Affinity Purification (TAP) Protocol. Modified from Rigaut et al. 1999. Univ.Washington Yeast Resource Center. - [Read Tandem Affinity Purification (TAP) Protocol]

TCA Acetone precipitation protocol DOC - http://caffeine.chemistry.montana.edu/mass-spec/TCA%20acetone%20ppt%20protocl..doc

Category: Protein Protocols: Protein Precipitation Procols

TCA Acetone precipitation protocol DOC. Matt Shipman. 2006. Adapted and modified from Gruehler et al., 2005. - [Read TCA Acetone precipitation protocol DOC]

Yeast IF without Dehydration - http://genomics.princeton.edu/botstein/protocols/non_MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF without Dehydration. Botstein Lab. Protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. - [Read Yeast IF without Dehydration]

Articles (1-1 of 1)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.