mixes Protocols

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for nick-translation labeling of ds DNA with nick translation mixes for in situ probes. Includes: Labeling reaction with DIG-dUTP or Biotin-dUTP; Labeling reaction with Fluorescein-dUTP, or Tetramethylrhodamine-dUTP; Purification of labeled probe. - [Read Nick-Translation Labeling of ds DNA with Nick Translation Mixes for In Situ Probes Protocol]

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes. Includes: PCR DIG labeling reaction for moderately labeled probes; PCR fluorescein labeling reaction for direct in situ probes.
- [Read PCR Labeling of Ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes]

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes. Includes: PCR DIG labeling reaction for highly labeled probes containing unique sequences; PCR DIG labeling reaction for moderately labeled probes; PCR fluorescein labeling reaction for direct in situ probes. - [Read PCR Labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes Protocol]

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Procedures for labeling DNA, RNA, and oligonucleotides with DIG, biotin, or fluorochromes. Includes: Random primed labeling of ds DNA with DIG-, Biotin- or Fluorescein-High Prime reaction mix; PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes. - [Read Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes]

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Procedures for labeling DNA, RNA, and oligonucleotides with DIG, Biotin, or Fluorochromes. Includes: Random primed labeling of ds DNA with DIG-, Biotinor Fluorescein-High Prime reaction mix; PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes. - [Read Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes]

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Digesting DNA with Restriction Endonucleases introduction. Analytical and Preparative reaction mixes. All about restriction enzymes. Pizzorno, BIOL 327. - [Read Restriction Enzyme Digestion of DNA]