mitotic Protocols

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Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols - http://www.fgsc.net/fgn48/Kaminskyj.htm

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

How to Measure Mitotic Instability of Segmental Duplications - http://www.fgsc.net/neurosporaprotocols/How%20to%20measure%20mitotic%20instability%20of%20segmental%20duplications.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to measure mitotic instability of segmental duplications. - [Read How to Measure Mitotic Instability of Segmental Duplications]

Mitotic Chromosome from Cell Cultures Protocol - http://www.jax.org/cyto/culture_prep.html

Category: Cytogenetics Protocols

Protocol for mitotic chromosome from cell cultures. Includes: Colchicine Treatment; Monolayer cultures; Cell Suspensions; Cell Harvest; Chromosome Slide Preparation. - [Read Mitotic Chromosome from Cell Cultures Protocol]

Mitotic Chromosome Preparations from Bone Marrow Protocol - http://www.jax.org/cyto/marrow_preps_alt.html

Category: Cytogenetics Protocols

Protocol for mitotic chromosome preparations from bone marrow. - [Read Mitotic Chromosome Preparations from Bone Marrow Protocol]

Mitotic Chromosome Preparations from Mammalian Suspension Cell Cultures Protocol - http://www.le.ac.uk/bl/phh4/promitotic.htm

Category: Cytogenetics Protocols

Protocol for mitotic chromosome preparations from mammalian suspension cell cultures. - [Read Mitotic Chromosome Preparations from Mammalian Suspension Cell Cultures Protocol]

Protocol Spindle Assembly In Vitro - http://www.bio.com/protocolstools/protocol.jhtml?id=p1051

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes an in vitro reaction to assay mitotic spindle assembly. The assay uses Cytostatic Factor extract made from Xenopus eggs, fluorescently-labelled tubulin, and prepared sperm nuclei. Spindle assembly is monitored by immunofluorescence microscopy. - [Read Protocol Spindle Assembly In Vitro]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cytogenetics Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Prtocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Prtocol]