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Gentle lysis of cells in sucrose-containing Mito-Buffer - http://www.celldeath.de/apometh/mitobuff.html

Category: Cell Biology and Cell Culture: Cell Lysis Protocols

Gentle lysis of mammalian cells for cytochrome c release assay. Based on Yang et al., 1997 Celldeath.de - [Read Gentle lysis of cells in sucrose-containing Mito-Buffer]

Gentle lysis of mammalian cells for cytochrome c release assay - http://www.celldeath.de/apometh/mitobuff.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Lysis in the sucrose-containing Buffer ("Mito-Buffer") is supposed to prevent accidential disrupture of the mitochondria to prevent the leakage of mitochondrial proteins (such as cytochrome c) into the cytosol. Celldeath.de - [Read Gentle lysis of mammalian cells for cytochrome c release assay]