mirmira Protocols

Category: DNA Protocols: EMSA DNA-Protein Protocols

There are multiple variations to this protocol, but they find that this one works well in all cases tested. Mirmira Lab. - [Read General EMSA Protocol]

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Mirmira Lab ChIP Protocol. 50-step protocol forformaldehyde crosslinking to ChIP and also provides recipes for all buffer reagents. - [Read Mirmira Lab ChIP Protocol]

Category: Protein Protocols: Nuclear Extraction Protocols

Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia - [Read Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots]

Category: DNA Protocols: EMSA DNA-Protein Protocols

Creation of Probe for EMSA using Oligonucleotide annealing, p-32, and T4 Polynucleotide Kinase. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assay]

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for radiolabeling of probes for electrophoretic mobility shift assays. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assays Protocol]

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]