miniprep Protocols

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DNA cloning Procedures - http://hulab.tamu.edu/Protocols/cloning/

Agarose gel purification, Annealing and extending, oligonucleotides, Ethanol Precipitation, Ligation Miniprep, Oligonucleotide purification, Recovering DNA bands, Restriction digest Gene Clean. The Hu Lab. - [Read DNA cloning Procedures]

DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm

Category: BACs Bacterial Artificial Chromosome Protocols

Rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol]

DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol]

DNA Microprep Isolation from Plants Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p9010

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the advantage of isolating DNA from plant material very rapidly. The procedure requires a table-top drill-press (mechanized homogenizer). - [Read DNA Microprep Isolation from Plants Protocol]

DNA Miniprep Isolation from Plants Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1079

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

The original maize DNA miniprep protocol is used extensively for many plant species and different tissues. This slightly modified version is acceptable for most DNA extractions. The procedure has the advantage of speed and its use of inexpensive reagents. - [Read DNA Miniprep Isolation from Plants Protocol]

Eppendorf Miniprep protocol - http://wolverton.owu.edu/lab/2006/01/eppendorf-miniprep-protocol

Category: Microbiology: Mini-Prep Plasmid Purification

Detailed Protocol for Eppendorf Miniprep protocol. Wolverton Lab - [Read Eppendorf Miniprep protocol]

Methods for DNA isolation - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/DNAisol.html

Category: DNA Protocols: DNA Extraction Protocols

Large scale double-stranded DNA isolation, Miniprep double-stranded DNA isolation, Large scale M13RF isolation, Single-stranded M13 DNA isolation using phenol. Minion Lab.Veterinary Medicine Iowa Univ. - [Read Methods for DNA isolation]

Yeast DNA Isolation: Miniprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4148

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of DNA from a 5-ml liquid culture of yeast. - [Read Yeast DNA Isolation: Miniprep Protocol]

Yeast Miniprep (Plasmid Rescue) Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastrescue.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast miniprep (plasmid rescue). - [Read Yeast Miniprep (Plasmid Rescue) Protocol]

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Purifying DNA Fragments from Agarose Gels Alternate Protocol

An alternate protocol for purifying DNA fragments from agarose gels.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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