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Affinity Antibody Purification of Protein A/G Chromatography - http://www.upstate.com/misc/protocol_detail.q.prot.e.antibody_purification.a.name.e.Antibody_Purification

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Antibody Purification (Antiserum or Ascites by Protein A/G Chromatography). Species and Type of Antibody Agarose Rabbit IgG Protein A or Protein G Mouse IgG1 Protein G Mouse IgG2 Protein A or Protein G Mouse IgG3 Protein G Sheep IgG Protein G but binds weakly Rat IgG Protein G but binds weakly Guinea Pig IgG Protein A Dog IgG Protein A Goat IgG Protein G Pig IgG Protein A Hamster IgG Protein G. By Millipore. - [Read Affinity Antibody Purification of Protein A/G Chromatography]

Chromatin Immunoprecipitation (ChIP) Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.chrom_ip.a.name.e.Chromatin_Immunoprecipitation_Assay

Category: Epigenetics and Methylation Protocols: ChIP Chromatin Immunoprecipitation Protocols

Chromatin Immunoprecipitation (ChIP) Assay protocol. Millipore. Includes protocols on Optimization of DNA Shearing, Sonication and PCR for ChIP. - [Read Chromatin Immunoprecipitation (ChIP) Assay]

Microbial Detection in Ice Tea Using the Millipore Milliflex Rapid Microbiology Detection System - http://www.millipore.com/techpublications/tech1/an1019en00

Category: Bacterial Protocols

Ice tea has a complex composition, which leads to reduced filterability, and a decrease in sample throughput. Its composition can generate background or false positive signals. It is also well known that ice tea contains molecules that can inhibit the bioluminescence reaction, which can generate false negative results. The aim of this study was to develop a protocol that was able to neutralize these affects and enable faster detection of contamination. - [Read Microbial Detection in Ice Tea Using the Millipore Milliflex Rapid Microbiology Detection System]

Millipore ZipTip detailed protocol. MSCLS - http://www.cbs.umn.edu/msp/protocols/ziptip.shtml

Category: Proteomic Protocols

ZipTip Protocol detailed protocol. MSCLS Center for Mass Spectrometry and Proteomics - [Read Millipore ZipTip detailed protocol. MSCLS]

Millipore's ZipTip Protocol - http://www.millipore.com/publications.nsf/docs/tn072

Category: Mass Spectrometry Protocols

Millipore's ZipTip Protocol. Intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. Millipore. - [Read Millipore's ZipTip Protocol]

Protocol: Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters - http://www.millipore.com/techpublications/tech1/6dlk4v

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

In vitro transcription reactions employing T3, T7 or SP6 phage-encoded RNA polymerases are widely used to synthesize RNA from recombinant vectors containing appropriate promoters. Production of large amounts of specific RNA is valuable in the preparation of hybridization probes and in vitro translation studies; in the synthesis of ribozymes, rRNA, SRP, antisense RNA and substrates for RNA splicing; and in RNA-protein interaction studies. - [Read Protocol: Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters]

Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters Protocol - http://www.millipore.com/techpublications/tech1/6dlk4v

Category: Nucleic Acid Purification Protocols

Protocol for purification of in vitro synthesized mRNA with Microcon or Centricon Centrifugal Filters. - [Read Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters Protocol]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.