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Transient Transfection Protocol: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V2.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection protocol for the ecotropic/amphotropic producer line including titering and concentration of viral stocks. - [Read Transient Transfection Protocol: Viral Production]

Transient Transfection: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V1.html

Category: Transfection Protocols: Transient Transfection Protocols

This transient transfection protocol for the ecotropic/amphotropic producer line includes instructions for titering and concentration of viral stocks. This procedure gives consistently higher transfection efficiency. - [Read Transient Transfection: Viral Production]

Tyramide Amplification and Synthesis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T4.html

Category: Immunohistochemistry Protocols

Protocol provides the sensitivity of enzymatic detection (HRP) in immunohistochemical procedures with the versatility and convenience of fluorescence detection. - [Read Tyramide Amplification and Synthesis Protocol]

Use of the Lab Micropipette - http://www.biotech.iastate.edu/publications/ppt_presentations/html/DNAMicropipetteUseage/01.html

Category: General Research Protocols and Methods

Use of the Lab Micropipette. Mike Zeller - [Read Use of the Lab Micropipette]

Western Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/W1.html

Category: Western Blot Protocols: General Western Blot Methods Protocols

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system. - [Read Western Blot Protocol]

[3H]Thymidine Labeling Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T3.html

Category: Cell Culture Protocols

Protocol on [3H]Thymidine labeling. Protocol includes: Explant Culture Medium; 100X (cold) Thymidine; Dektol Developer; Fixer - [Read [3H]Thymidine Labeling Protocol]

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